Quality Level
form
powder blend
pH
6.8-7.2 (20-25 °C, 263 mg/L in water)
solubility
water: 263 mg/mL, clear, colorless
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
General description
SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.
Application
Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
- Preparing nucleic acids for hybridization
- Hybridization buffer for Northern and Southern blots
- Washing buffer for Northern and Southern blots
Features and Benefits
- Multi-purpose use in hybridizations
- Easily reconstituted and diluted to any concentration needed
Preparation Note
Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.
저장 등급
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
프로토콜
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Protocol for the Preparation of Arabidopsis Meiotic Chromosome Spreads and Fluorescent in situ Hybridization
Bolanos V, et al.
The Plant Journal (2013)
AUTEN-67, an autophagy-enhancing drug candidate with potent antiaging and neuroprotective effects
Papp D, et al.
Autophagy, 12(2), 273-286 (2016)
M Böttger et al.
Nucleic acids research, 9(20), 5253-5268 (1981-10-24)
Complexes of histones H1 with superhelical SV40 DNA obtained by direct mixing were studied in 0.1 SSC buffer corresponding to 0.02 M Na+. Depending on the molar input ratio H1/DNA three classes of sedimenting species were observed: (1) a component
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| S0902-1L | 04061836830960 |
| S0902-5X1L | 04061838175182 |