H7033
PerfectHyb™ Plus Hybridization Buffer
for Northern and Southern blotting, solution
동의어(들):
hyb buffer, hyb solution, hybridization solution, northern hybridization buffer, southern hybridization buffer
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.25
UNSPSC Code:
12161700
General description
Sigma′s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.
Application
Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.
Features and Benefits
- Fast and easy-to-use
- Up to 20x more sensitive than conventional buffers
- Doesn′t require blocking steps
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent, colorimetric , and DIG detection assays
- Compatible with radioactive and non-radioactive probes
- Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes
Other Notes
A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.
Legal Information
PerfectHyb is a trademark of Sigma-Aldrich Co. LLC
저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
프로토콜
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Sivaramakrishnan Muthuswamy et al.
Planta, 233(1), 201-208 (2010-09-28)
Protein sumoylation plays an important role in plant development, flowering-time regulation, and abiotic stress response. However, the molecular role of sumoylation in these pathways is largely unknown. It was shown previously that in mutants of the inner nuclear basket nucleoporin
Yinping Jiao et al.
BMC plant biology, 11, 141-141 (2011-10-22)
miRNAs are known to play important regulatory roles throughout plant development. Until recently, nearly all the miRNAs in maize were identified by comparative analysis to miRNAs sequences of other plant species, such as rice and Arabidopsis. To find new miRNA
Edyta Koscianska et al.
TheScientificWorldJournal, 11, 102-117 (2011-01-11)
This protocol describes how to perform northern blot analyses to detect microRNAs and their precursors with single-nucleotide resolution, which is crucial for analyzing individual length variants and for evaluating relative quantities of unique microRNAs in cells. Northern blot analysis consists
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| H7033-1L | 04061833803097 |
| H7033-125ML | 04061833803080 |
| H7033-50ML | 04061833803165 |