S6639
SSC Buffer 20× Concentrate
for Northern and Southern blotting, solution
동의어(들):
20X SSC Buffer, SSC hybridization buffer, Saline-sodium citrate buffer
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.25
UNSPSC Code:
41105319
grade
Molecular Biology
Quality Level
description
Saline-Sodium Citrate buffer, made with ultrapure water
sterility
0.2 μm filtered
form
solution
color
colorless
pH
6.9 —7.1
application(s)
diagnostic assay manufacturing
foreign activity
DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected
storage temp.
room temp
General description
SSC (Saline Sodium Citrate) Buffer 20x liquid is a standard reagent in Southern and Northern hybridization procedures. It is used to control stringency of wash buffer for the washing steps after hybridization.
Application
SSC Buffer 20× Concentrate has been used
- as a hybridization buffer on in situ hybridization human-specific repetitive Alu sequence
- as a washing buffer for membrane for Southern hybridization, dot blot of mice genomic DNA and northern blotting of microRNA
- as an incubation buffer in fluorescence in situ hybridization (FISH) of L1 retrotransposon
Features and Benefits
- Convenient ready-to-use liquid
- Multi-purpose use in hybridizations
Preparation Note
SSC Buffer 20x contains 3M NaCl in 0.3M sodium citrate (pH 7.0).
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
프로토콜
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
문서
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
사용 가능한 형광 동소 교잡법(FISH) 절차, 시약, 장비.
Chronic hypoxia facilitates Alzheimer's disease through demethylation of gamma-secretase by downregulating DNA methyltransferase 3b
Liu H, et al.
Alzheimers Dement., 12(2), 130-143 (2016)
L1 retrotransposon expression in circulating tumor cells
Papasotiriou I, et al.
Testing, 12(2), e0171466-e0171466 (2017)
Michaela Asp et al.
Scientific reports, 7(1), 12941-12941 (2017-10-13)
Heart failure is a major health problem linked to poor quality of life and high mortality rates. Hence, novel biomarkers, such as fetal marker genes with low expression levels, could potentially differentiate disease states in order to improve therapy. In
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| S6639-1L | 04061838258816 |