General description
Sigma′s Prehybridization Solution is a 2x ready-to-use buffer for Northern and Southern blot protocols.
Application
Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols.
Preparation Note
Sigma′s 2X Prehybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
- 200 μg/mL DNA
- 2mg Bovine Serum Albumin (BSA)
- 2mg Ficoll
- 2mg Polyvinylpyrrolidone (PVP)
- 0.15 M sodium citrate
- 1.5M NaCl
저장 등급
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
프로토콜
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Elizabeth S Colombo et al.
Investigative ophthalmology & visual science, 48(4), 1793-1800 (2007-03-29)
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T M Lau et al.
Human reproduction (Oxford, England), 11(12), 2629-2634 (1996-12-01)
The expression of endometrial progesterone receptor mRNA during the human menstrual cycle and in Norplant users was studied using digoxigenin-labelled ribonucleic probes for in-situ hybridization on 6 microns paraffin embedded endometrial sections. The staining intensity was scored blind semi-quantitatively. Blood
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 9-9 (1989)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| P1415-50ML | 04061834354949 |
| P1415-5X50ML | 04061834354970 |