grade
Molecular Biology
Quality Level
product line
BioReagent
form
solution
technique(s)
Southern blotting: suitable
impurities
DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected
pH
7.0±0.2
density
1.16 g/mL at 20 °C
storage temp.
room temp
General description
SSC Buffer 20x Concentrate is a standard reagent in Southern and Northern blotting procedures. This concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.
Application
Suitable for:
- Preparing nucleic acids for hybridization
- Hybridization buffer for Northern and Southern blots
- Washing buffer for Northern and Southern blots
Features and Benefits
- Convenient ready-to-use liquid
- Multi-purpose use in hybridizations
- Easily diluted to any concentration needed
Preparation Note
SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.
저장 등급
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
프로토콜
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
문서
Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.
Guanghua Yang et al.
BMC biology, 11, 86-86 (2013-07-23)
Human pluripotent stem cells (hPSCs) hold great promise for applications in regenerative medicine. However, the safety of cell therapy using differentiated hPSC derivatives must be improved through methods that will permit the transplantation of homogenous populations of a specific cell
Sterghios A Moschos et al.
BMC genomics, 8, 240-240 (2007-07-21)
At present, nothing is known of the role of miRNAs in the immune response in vivo despite the fact that inflammation is thought to underlie multiple acute and chronic diseases. In these circumstances, patients are commonly treated with corticosteroids such
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 93017-10L-F | 04061833271360 |