grade
Molecular Biology
product line
BioReagent
form
lyophilized powder
impurities
DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected
suitability
suitable for nucleic acid hybridization
storage temp.
2-8°C
General description
Denhardt′s solution is a mixture of high-molecular weight polymers that act as blocking agents. It is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.
Application
Suitable to block non-specific binding sites in western, northern and Southern blot membranes
Preparation Note
To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at −20°C for up to two years.
Other Notes
Each vial of Denhardt′s powder contains 50 mG Bovine Serum Albumin (BSA), 50 mG Ficoll, and 50 mG Polyvinylpyrrolidone (PVP).
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
J. Sambrook et al.
Molecular Cloning: A Laboratory Manual (1989)
Flavien Pillet et al.
Nucleic acids research, 39(17), 7477-7486 (2011-06-10)
The segregation of plasmid F of Escherichia coli is highly reliable. The Sop partition locus, responsible for this stable maintenance, is composed of two genes, sopA and sopB and a centromere, sopC, consisting of 12 direct repeats of 43 bp. Each
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 30915-5ML | 04061826675359 |
| 30915-25ML | 04061826675342 |