grade
Molecular Biology
Quality Level
product line
BioReagent
form
powder
technique(s)
electrophoresis: suitable
pH
6.8-7.2(reconstituted per directions)
solubility
H2O: soluble, faintly yellow
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
room temp
General description
MOPS – EDTA – Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum resolution.
Application
Suitable for use when making formaldehyde-agarose gels and associated running buffer for RNA electrophoresis.
Preparation Note
1x MESA buffer contains:
- 40 mM MOPS
- 10 mM sodium acetate
- 1 mM EDTA (pH 8.3)
Dissolve entire contents of bottle in a final volume of 1L molecular biology grade water. This produces a 10x MESA stock solution that can be further diluted as needed.
저장 등급
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
프로토콜
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
Kathryn M Jones et al.
Journal of bacteriology, 184(9), 2491-2499 (2002-04-12)
Two operons have been cloned from Anabaena sp. strain PCC 7120 DNA, each of which encodes the three core subunits of distinct mitochondrial-type cytochrome c oxidases. The two operons are only 72 to 85% similar to one another at the
Xueyu Xiang et al.
Developmental biology, 275(2), 343-355 (2004-10-27)
Recently, we cloned and sequenced the cDNA of allurin, a sperm chemoattractant isolated from the jelly of Xenopus laevis eggs [Proc. Natl. Acad. Sci. U.S.A. 78 (2001) 11205]. In this report, we demonstrate that allurin mRNA is expressed almost exclusively
Molecular techniques for detection of gene expression in neuroimmunology.
Cowan, E.P., and Dhib-Jalbut, S.S.
Methods in Neuroscience, 24, 41-41 (1995)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| M5755-1L | 04061834060314 |