R6148
RNase A solution
Synonym(s):
GenElute™ RNAse A
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
NACRES:
NA.52
UNSPSC Code:
12352204
Specific activity:
1500 units/mL, 3000 units/mL
Biological source:
bovine pancreas
Concentration:
20 mg/mL in Tris buffer
biological source
bovine pancreas
Quality Level
form
liquid
specific activity
1500 units/mL, 3000 units/mL
concentration
20 mg/mL in Tris buffer
technique(s)
DNA purification: suitable
storage temp.
room temp
Gene Information
bovine ... RNASE1(282340), RNASE1(280930)
General description
Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute™ kits.
For sample preparation in GenElute™ kits.
Application
RNase A solution has been used:
- in apoptosis assay of Hep-2 cells
- for the colorimetric quantification of methylated DNA
- for DNA and RNA extraction
- in DNA laddering analysis
- in cell cycle analysis
- as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry
Biochem/physiol Actions
Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.
Preparation Note
For usage requirements, please refer to the kit procedure.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
Still not finding the right product?
Explore all of our products under RNase A solution
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Determination of the presence of 5-methylcytosine in Paramecium tetraurelia
Singh A, et al.
PloS one, 13(10), e0206667-e0206667 (2018)
Abdou ElSharawy et al.
Oncotarget, 7(46), 75353-75365 (2016-09-30)
The emerging potential of miRNAs as biomarkers for cancer detection demands parallel evaluation of strategies for reliable identification of disease-related signatures from easily accessible and pertinent body compartments. Here, we addressed whether efficient concentration of circulating miRNA-carrying particles is a
Phenotypic plasticity and biogeographic variation in physiology of habitat-forming seaweed: response to temperature and nitrate
Flukes EB, et al.
Journal of Phycology, 51(5), 896-909 (2015)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| R6148-.2ML | 04061837302992 |
| R6148-.6ML | 04061837303029 |
| R6148-1.7ML | 04061837303081 |
| R6148-25ML | 04061836695781 |
| R6148-2ML | 04061837303135 |
| R6148-4ML | 04061837303159 |
| R6148-8ML | 04061837303173 |
| R6148-.1ML | 04061837302954 |
| R6148-.25ML | 04061837302978 |
| R6148-.4ML | 04061837303012 |
| R6148-1.2ML | 04061837303043 |
| R6148-1.5ML | 04061837303067 |
| R6148-2.5ML | 04061837303104 |
| R6148-25ML-CO | 04061837841415 |