PLN350
GenElute™ Plasmid Miniprep Kit
sufficient for 350 purifications
Synonym(s):
GenElute™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute
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About This Item
NACRES:
NA.52
UNSPSC Code:
41105501
usage
sufficient for 350 purifications
Quality Level
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
test parameters
: <30 min kit run time, sample volume: 1-5 mL bacterial culture
greener alternative category
storage temp.
15-25°C
General description
The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
The kit has been used for the purification of plasmid DNA of Dirofilaria repens.
The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.
Features and Benefits
- Purified plasmid DNA in less than 30 minutes
- Purify up to 15 μg of plasmid DNA
- No detectable genomic DNA or RNA contamination
- No phenol/chloroform extraction or alcohol precipitation required
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Central nervous system
Storage Class
3 - Flammable liquids
wgk
WGK 2
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Dirofilaria repens: emergence of autochthonous human infections in the Czech Republic (case reports).
Mateju J
BMC Infectious Diseases, 16, 171-171 (2016)
Qifu He et al.
Frontiers in cell and developmental biology, 9, 747722-747722 (2021-10-19)
In this paper, on the basis of the differences in the hydrogen ion concentration (pH) of the diluent dairy goat semen on X/Y sperm motility, an X/Y sperm enrichment study was conducted to establish a simple and effective method for
Charlie Rowlands et al.
Scientific reports, 11(1), 20607-20607 (2021-10-20)
The development of computational methods to assess pathogenicity of pre-messenger RNA splicing variants is critical for diagnosis of human disease. We assessed the capability of eight algorithms, and a consensus approach, to prioritize 249 variants of uncertain significance (VUSs) that
Global Trade Item Number
| SKU | GTIN |
|---|---|
| PLN350-1KT | 04061836681326 |