Skip to Content
MilliporeSigma

Key Documents

View All Documentation

NA0200

GenElute™ HP Plasmid Midiprep Kit

greener alternative

sufficient for 25 purifications

Synonym(s):

GenElute™ HP Plasmid Midiprep Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

NACRES:
NA.52
UNSPSC Code:
41105501
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

usage

sufficient for 25 purifications

Quality Level

200

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

test parameters

sample volume: 1-5 mL bacterial culture

greener alternative category

, Aligned

storage temp.

15-25°C

General description

Click here to view the kit protocol
The GenElute HP Plasmid Midiprep Purification kits offer an ultrafast and efficient solution for large-scale plasmid preparation from E. coli cultures. These kits combine silica-based membrane technology and the convenience of spin or vacuum format. The kits also include a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis the DNA is bound to the silica membrane and contaminants are removed with a simple wash step. The DNA is then eluted in water or TE buffer by centrifugation.

The purified plasmid DNA is suitable for a wide variety of molecular biology applications, including restriction digestions and sequencing.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

GenElute™ HP Plasmid Midiprep Kit has been used to isolate and purify plasmids.

Features and Benefits

  • From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
  • Up to 350 μg of high-copy plasmid DNA
  • Flexibility of a vacuum or spin format
  • No phenol/chloroform extraction or alcohol precipitation required
  • Contains fewer plastic components, reducing the amount of waste

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • R1149
    Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )
    SDS
  • W0263
    Wash Solution 1
    SDS
  • W4639
    Wash Solution 2
    SDS
  • R6148
    RNase A solution
    SDS
  • C2112
    Column Preparation Solution
    SDS
  • L1912
    Lysis Solution
    SDS

signalword

Danger

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

wgk

WGK 3

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
086K6821
103K9292
103K9281
102K9283
074K6039

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

User Guide - NA0200
DNA & RNA Purification

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, tissue, blood, viruses, and other sample types.

Product Information Sheet
View All Related Content
Vanessa E Gray et al.
G3 (Bethesda, Md.), 9(11), 3683-3689 (2019-09-29)
Despite the importance of Aβ aggregation in Alzheimer's disease etiology, our understanding of the sequence determinants of aggregation is sparse and largely derived from in vitro studies. For example, in vitro proline and alanine scanning mutagenesis of Aβ40 proposed core
The distal upstream promoter in Ly49 genes, Pro1, is active in mature NK cells and T cells, does not require TATA boxes, and displays enhancer activity.
Gays F
Journal of Immunology, 194(12), 6068-6081 (2015)
Genome-wide CRISPR screens reveal a Wnt-FZD5 signaling circuit as a druggable vulnerability of RNF43-mutant pancreatic tumors.
Steinhart Z
Nature Medicine, 23(1), 60-68 (2017)
View All Related Papers

Global Trade Item Number

SKUGTIN
NA0200-1KT04061835573684