S8830
Protease Inhibitor Cocktail
SIGMA FAST, EDTA-Free, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use in purification of Histidine-tagged proteins
동의어(들):
Protease Inhibitor Cocktail
제품 이름
SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagged proteins
생물학적 소스
bovine lung
Quality Level
양식
tablet
저장 온도
2-8°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
일반 설명
SIGMAFAST™ Protease Inhibitor Cocktail Tablets, EDTA-Free is a water-soluble tablet formulation designed to inhibit serine, cysteine, aspartic and metalloproteases. The tablets contain no strong metal chelators, such as EDTA, and are compatible with sample processing by immobilized metal affinity chromatography (IMAC). The tablets also contain a unique, proprietary formulation that includes a pepstatin A salt for additional protection.
애플리케이션
The broad specificity of SIGMAFAST™ Protease Inhibitor Cocktail Tablets makes them suitable for use in the purification of histidine-tagged proteins and as a general-purpose protease inhibitor cocktail. The tablets can also be used as components of buffers for cell lysis, and have been optimized and tested for use with HIS-Select® media.
생화학적/생리학적 작용
Inhibits serine, cysteine, aspartic, and metalloproteases.
특징 및 장점
EDTA-Free: Contains no strong metal chelators, such as EDTA, which might interfere with IMAC processing.
Pepstatin A: Contains a unique, proprietary formulation that includes a pepstatin A salt for additional protection not normally obtained in a tablet format.
Broad Specificity: Inhibits serine, cysteine, aspartic, and metalloproteases, providing broad protection for protein samples.
High Yield: One tablet makes 100 mL of cocktail, and is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.
Long Stability: The tablets are stable as supplied for at least 2 years at 2–8 °C, and the reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.
Pepstatin A: Contains a unique, proprietary formulation that includes a pepstatin A salt for additional protection not normally obtained in a tablet format.
Broad Specificity: Inhibits serine, cysteine, aspartic, and metalloproteases, providing broad protection for protein samples.
High Yield: One tablet makes 100 mL of cocktail, and is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.
Long Stability: The tablets are stable as supplied for at least 2 years at 2–8 °C, and the reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.
물리적 형태
Tablet that is water soluble
제조 메모
One tablet makes 100 mL of cocktail. One tablet is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.
The tablets are stable as supplied for at least 2 years at 2–8 °C. The reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.
기타 정보
AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin
법적 정보
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1A
Storage Class Code
8A - Combustible corrosive hazardous materials
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
Katarzyna Rybak et al.
Developmental cell, 29(5), 607-620 (2014-06-03)
Plant cytokinesis is initiated in a transient membrane compartment, the cell plate, and completed by a process of maturation during which the cell plate becomes a cross wall. How the transition from juvenile to adult stages occurs is poorly understood.
Saponin of Momordica cymbalaria exhibits anti-inflammatory activity by suppressing the expression of inflammatory mediators in lipopolysaccharide-stimulated RAW264.7 macrophages
Samaddar S and Koneri R
Pharmacognosy Research (2019)
Estrogen-dependent disruption of intracellular iron metabolism augments the cytotoxic effects of doxorubicin in select breast and ovarian cancer cells
Bajbouj K, et al.
Cancer Management and Research (2019)
Jeremy Celver et al.
Journal of neurochemistry, 120(1), 56-69 (2011-11-01)
Detergent-resistant membranes (DRM) are thought to contain structures such as lipid rafts that are involved in compartmentalizing cell membranes. We report that the majority of D(2)-dopamine receptors (D(2)R) expressed endogenously in mouse striatum or expressed in immortalized cell-lines is found
The effect of pegylated granulocyte colony-stimulating factor treatment prior to experimental mastitis in lactating Holsteins
Powell EJ, et al.
Dairy science & technology (2018)
문서
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
관련 콘텐츠
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.