P1860
Protease Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, DMSO solution
동의어(들):
Protease Inhibitor Solution, protease inhibitor
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제품정보 (DICE 배송 시 비용 별도)
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C
제품 이름
Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution
Quality Level
form
DMSO solution
solubility
water: soluble
shipped in
dry ice
storage temp.
−20°C
General description
Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.
Application
Protease Inhibitor Cocktail has been used:
- as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
- to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
- in trypsin solution for trypsin-inhibited studies
- as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot
This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.
Biochem/physiol Actions
Inhibits serine, cysteine, aspartic proteases, and aminopeptidases
Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.
Features and Benefits
- Broad specificity
- Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
- Contains no metal chelators: Ensures compatibility with downstream applications
- Convenient packaging: 1 mL in glass bottle
- Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.
Physical form
Solution in DMSO (D 2650, Hybri-Max).
Preparation Note
Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.
Other Notes
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
Bestatin
E-64
Leupeptin
Pepstatin A
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
Disclaimer
After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
저장 등급
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
문서
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
관련 콘텐츠
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
Raymond L Warters et al.
Radiation research, 172(1), 82-95 (2009-07-08)
Although skin is usually exposed during human exposures to ionizing radiation, there have been no thorough examinations of the transcriptional response of skin fibroblasts and keratinocytes to radiation. The transcriptional response of quiescent primary fibroblasts and keratinocytes exposed to from
Takuma Shiratori et al.
Journal of immunology (Baltimore, Md. : 1950), 200(1), 218-228 (2017-11-17)
As osteoclasts have the central roles in normal bone remodeling, it is ideal to regulate only the osteoclasts performing pathological bone destruction without affecting normal osteoclasts. Based on a hypothesis that pathological osteoclasts form under the pathological microenvironment of the
Matthew Townsend et al.
The Journal of physiology, 572(Pt 2), 477-492 (2006-02-14)
The accumulation of amyloid beta-protein (Abeta) in brain regions serving memory and cognition is a central pathogenic feature of Alzheimer's disease (AD). We have shown that small soluble oligomers of human Abeta that are naturally secreted by cultured cells inhibit
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| P1860-1ML | 04061838434128 |