P1860
Protease Inhibitor Cocktail
DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, DMSO solution
동의어(들):
Protease Inhibitor Solution, protease inhibitor
제품 이름
Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution
Quality Level
양식
DMSO solution
solubility
water: soluble
배송 상태
dry ice
저장 온도
−20°C
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일반 설명
Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension
The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.
애플리케이션
Protease Inhibitor Cocktail has been used:
- as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
- to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
- in trypsin solution for trypsin-inhibited studies
- as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot
This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.
생화학적/생리학적 작용
Inhibits serine, cysteine, aspartic proteases, and aminopeptidases
Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.
특징 및 장점
- Broad specificity
- Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
- Contains no metal chelators: Ensures compatibility with downstream applications
- Convenient packaging: 1 mL in glass bottle
- Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.
물리적 형태
Solution in DMSO (D 2650, Hybri-Max).
제조 메모
Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.
기타 정보
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A
Bestatin
E-64
Leupeptin
Pepstatin A
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
면책조항
After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
188.6 °F
Flash Point (°C)
87 °C
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문서
While aprotinin and bovine pancreatic trypsin inhibitor (BPTI) are the same protein sequence, the term aprotinin is typically used when describing the protein derived from bovine lung.
관련 콘텐츠
Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.
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