MITOISO1
Mitochondria Isolation Kit
sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues
동의어(들):
Mitochondria Purification Kit
Quality Level
사용
sufficient for 10-20 g (animal tissue)
sufficient for 50 assays (2 mL)
유통기한
2 yr
기술
fractionation: suitable
배송 상태
dry ice
저장 온도
−20°C
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애플리케이션
Mitochondria Isolation Kit has been used:
- for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity
- for the isolation of mitochondria from glomeruli and heart
- for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential
The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.
특징 및 장점
- Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
- Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
- Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
- Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane
키트 구성품 역시 별도로 이용 가능함
제품 번호
설명
SDS
- T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solidSDS
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
10 - Combustible liquids
Mitochondrial dysfunction mediates aldosterone-induced podocyte damage: a therapeutic target of PPARgamma
Zhu C, et al.
The American Journal of Pathology, 178(5), 2020-2031 (2011)
DJ-1 protects the heart against ischemia-reperfusion injury by regulating mitochondrial fission
Shimizu Y, et al.
Journal of Molecular and Cellular Cardiology, 97, 56-66 (2016)
Young-Kyoung Shin et al.
Cancer research, 65(8), 3162-3170 (2005-04-19)
5-Fluorouracil (5-FU) is widely used for treatment of advanced colorectal cancer. However, it is common for such patients to develop resistance to 5-FU, and this drug resistance becomes a critical problem for chemotherapy. The mechanisms underlying this resistance are largely
Enrique Santamaria et al.
Proceedings of the National Academy of Sciences of the United States of America, 100(6), 3065-3070 (2003-03-13)
Recent work shows that S-adenosylmethionine (AdoMet) helps maintain normal liver function as chronic hepatic deficiency results in spontaneous development of steatohepatitis and hepatocellular carcinoma. The mechanisms by which these nontraditional functions of AdoMet occur are unknown. Here, we use knockout
Mithu Guha et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 20(8), 1224-1226 (2006-04-11)
Hepatic dysfunction is a common clinical complication in malaria, although its pathogenesis remains largely unknown. Using a variety of in vivo and ex vivo approaches, we have shown for the first time that malarial infection induces hepatic apoptosis through augmentation
문서
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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