MITOISO3
Yeast Mitochondria Isolation Kit
sufficient for 40 applications (using 20 OD culture preparations), isolation of an enriched mitochondrial fraction from yeast cells
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.32
UNSPSC Code:
12352200
Quality Level
usage
sufficient for 40 applications (using 20 OD culture preparations)
storage temp.
−20°C
General description
Mitochondria, the site of most of the energy production in eukaryotic cells, are characterized by a double membrane structure, an outer membrane and folded inner membrane. Isolated mitochondria may be used as tools for in vitro studies such as respiration and energy production studies, apoptosis, mtDNA and mtRNA, and mitochondrial protein profiling.
Application
The kit contains all the reagents required for yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation. In addition, the kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies and a storage buffer for use with intact mitochondria. Yeast Mitochondria Isolation Kit has been used in the isolation and extraction of mitochondria.
Features and Benefits
• The kit′s features include speed, sensitivity, ease of use, and specificity
• The kit contains all the reagents required for a fast and easy yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation
• The kit provides two alternatives for cell membrane disruption. The first one by a detergent and second one by homogenization.
• The kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies
• The kit includes a storage buffer for use with intact mitochondria.
• The reagents are sufficient for 40 procedures using 20 OD culture preparations
• The kit was tested on Saccharomyces cerevisiae, Pichia pastoris, and Schizosaccharomyces pombe
• The kit contains all the reagents required for a fast and easy yeast cell wall lysis by lyticase (spheroplast formation) followed by cell membrane lysis/breakage and mitochondria isolation
• The kit provides two alternatives for cell membrane disruption. The first one by a detergent and second one by homogenization.
• The kit includes an extraction buffer for mitochondrial protein profiling to be used in proteome studies
• The kit includes a storage buffer for use with intact mitochondria.
• The reagents are sufficient for 40 procedures using 20 OD culture preparations
• The kit was tested on Saccharomyces cerevisiae, Pichia pastoris, and Schizosaccharomyces pombe
signalword
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 2
저장 등급
10 - Combustible liquids
wgk
WGK 3
문서
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
Christian Streng et al.
The EMBO journal, 40(17), e108083-e108083 (2021-07-14)
Mitochondria are essential organelles because of their function in energy conservation. Here, we show an involvement of mitochondria in phytochrome-dependent light sensing in fungi. Phytochrome photoreceptors are found in plants, bacteria, and fungi and contain a linear, heme-derived tetrapyrrole as
Yeast cells expressing the human mitochondrial DNA polymerase reveal correlations between polymerase fidelity and human disease progression
Qian Y, et al.
The Journal of Biological Chemistry (2017)
Erika Steele et al.
Microbial cell factories, 20(1), 138-138 (2021-07-21)
Myo-Inositol Phosphate Synthase (MIP) catalyzes the conversion of glucose 6- phosphate into inositol phosphate, an essential nutrient and cell signaling molecule. Data obtained, first in bovine brain and later in plants, established MIP expression in organelles and in extracellular environments.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| MITOISO3-1KT | 04061834066798 |