Quality Level
form
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
Earle’s salts (5% CO2): yes
NaHCO3: no
phenol red: yes
L-glutamine: yes
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
It contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser. May be preferred for growth of adherent cells in the presence of reduced serum or as a base for development of a serum free medium.
Minimum Essential Medium Eagle consists of inorganic salts, water-soluble vitamins, sugar, and essential amino acids. It is widely used as a media formulation for many cell culture studies. It is a modified version of Basal medium Eagle (BME) and the concentrations of the majority of amino acids are twice as compared to BME.
Application
Minimum Essential Medium Eagle has been used to culture osteoblast-like cell line MG-63, dispersed ventricular cells, and endometrial adenocarcinoma Ishikawa cells.
Preparation Note
Formulated to contain 9.6 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.
저장 등급
11 - Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
문서
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.
Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google
Erika Nahomy Marino-Marmolejo et al.
BMC microbiology, 16(1), 290-290 (2016-12-14)
Pharmaceutical industry demands innovation for developing new molecules to improve effectiveness and safety of therapeutic medicines. Preclinical assays are the first tests performed to evaluate new therapeutic molecules using animal models. Currently, there are several models for evaluation of treatments
Tatsuma Yao et al.
Reproductive medicine and biology, 16(2), 99-117 (2017-12-21)
Cell culture technology has spread prolifically within a century, a variety of culture media has been designed. This review goes through the history, characteristics and current issues of animal-cell culture media. A literature search was performed on PubMed and Google
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| M0643-50KG | 04061834408901 |
| M0643-10L | 04061833993750 |
| M0643-50K | 04061834181491 |
| M0643-25KG | 04061834408895 |
| M0643-10X1L | 04061835568802 |
| M0643-1L | 04061837459504 |
| M0643-50L | 04061833993767 |