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Merck

F2442

Fetal Bovine Serum

USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

동의어(들):

Fetal Bovine Sera, Fetal Calf Serum, FBS, FCS, sera, serum

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352207
eCl@ss:
32160409
NACRES:
NA.71
MDL number:
Biological source:
bovine fetus
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
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도움 문의


biological source

bovine fetus

Quality Level

sterility

sterile-filtered

product line

FBS Classic

composition

Bovine IgG, ≤1 mg/mL , Hemoglobin, ≤20 mg/dL

origin

USA origin

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

impurities

≤10 EU/mL endotoxin

shipped in

dry ice

storage temp.

−20°C

General description

Fetal Bovine Serum (FBS) is a complex mixture with low and high molecular weight biomolecules exhibiting optimal growth-enhancing and growth-suppressing functions. FBS contains molecules, such as growth factors, proteins, vitamins, trace elements, hormones etc. These molecules are important for the growth and maintenance of cells. It is a common supplement for cell culture media. FBS is derived from the calf blood. It is devoid of fibrin and clotting factors. Heat inactivation of FBS blocks the complement system. FBS derived from clotted blood is the most widely used undefined supplement in eukaryotic, especially mammalian, cell culture. It is typically used with the classical media that were developed in the 1950s and 1960s. These include many popular media such as Minimum Essential Medium (MEM), Dulbecco′s Modified Eagles Media (DME, DMEM); Iscove′s Modified Eagles Medium (IMDM), M199 and Roswell Park Memorial Institute medium, RPMI-1640.

Application

Fetal Bovine Serum has also been used as a component of:
  • perivascular-resident macrophage-like melanocytes (PVM/M) growth medium
  • synthetic oviduct fluid (SOF)
  • Eagle′s minimum essential medium (EMEM)
  • neurobasal medium
  • Hyclone McCoys 5 A medium
  • Hanks medium

Biochem/physiol Actions

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Analysis Note

  • Endotoxin and hemoglobin tested
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Other Notes

Tested for the presence of bacteria, virus, mycoplasma, endotoxin and for hemoglobin content. Triple filtered with 0.1 micron membrane under aseptic conditions.


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저장 등급

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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문서 라이브러리 방문


프로토콜

Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.

Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

문서

High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.

Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.

High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.

모든 기사 보기

관련 콘텐츠

Learn how to effectively filter fetal bovine serum (FBS) using Stericup® Quick Release vacuum filtration devices, including flow test data and analysis.

Step-by-step protocol for generating apical-out human gut organoids for microbiome, ADME/Tox, viral and gastrointestinal related disease research. See the complete organoid culture protocol.


Axenic Procyclic Culture of L. tropica and L. donovani in Culture of FBS-free Medium
Mosa FM, et al.
Ife Journal of Science, 4B, 3088-3093 (2015)
MENT, a heterochromatin protein that mediates higher order chromatin folding, is a new serpin family member.
Grigoryev SA et al.
The Journal of Biological Chemistry, 274, 5626-5626 (1999)
Inhibiting tyrosine phosphorylation of protein kinase Cd (PKCd) protects the salivary gland from radiation damage.
Wie SM et al.
The Journal of Biological Chemistry, 289, 10900-10900 (2014)



국제 무역 품목 번호

SKUGTIN
F2442-100ML04061838338709
F2442-1L04061837034589
F2442-24X500ML04061838255372
F2442-50ML04061838255396
F2442-500ML04061838255389
F2442-6X500ML04061838255402