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Merck

M0268

Minimum Essential Medium Eagle

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

동의어(들):

EMEM, MEM

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제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.75
UNSPSC Code:
12352207
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form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.

Application

Minimum Essential Medium Eagle has been used:
  • in the isolation and culture of primary hepatocytes
  • to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
  • to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells

Features and Benefits

  • Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
  • The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.

Preparation Note

Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

Other Notes

Essentially the same as the original formula, except it is provided as a powder.


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저장 등급

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문


문서

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.


T L Freeman et al.
Biochemical pharmacology, 68(2), 223-230 (2004-06-15)
Activation of hepatic stellate cells (HSCs) involves the induction of ECM protein synthesis and rapid cell proliferation. Thus, agents that interfere with either process could potentially mitigate the development of liver disease by reducing the synthesis of proteins associated with
Jifu Mao et al.
ACS nano, 11(10), 10409-10416 (2017-09-08)
The poor mechanical properties and processability of pristine heterocyclic conductive polymers represent the most notable scientific and technological challenges that have greatly limited the application of these polymers. We report a soft and mechanically processable free-standing pristine polypyrrole (PPy) membrane
Xiaolong Sun et al.
Acta histochemica, 122(8), 151649-151649 (2020-11-10)
As one of the most frequent diabetic complications, diabetic foot ulcer (DFU) can cause limb ischemia or even amputation. Paeoniflorin (PF) has been reported to possess many kinds of biological functions, such as antioxidant and anti-inflammatory effects. However, the role



국제 무역 품목 번호

SKUGTIN
M0268-25KG04061834408819
M0268-50KG04061834408826
M0268-10L04061835568772
M0268-10X1L04061835568789
M0268-1L04061837473128
M0268-50L04061833992906