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Merck

G7646

β-Glucuronidase from Escherichia coli

Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)

동의어(들):

β-D-Glucuronide glucuronosohydrolase

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
EC 번호:
Specific activity:
5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
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도움 문의


type

Type VII-A

Quality Level

form

lyophilized powder

specific activity

5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)

mol wt

290 kDa

composition

Protein, ~25% biuret

storage temp.

−20°C

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Contains buffer salts and stabilizer.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).


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pictograms

Health hazard

signalword

Danger

저장 등급

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

hcodes

Hazard Classifications

Resp. Sens. 1

wgk

WGK 1



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문서 라이브러리 방문


프로토콜

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

문서

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

관련 콘텐츠


Optimized conditions for the emzymatic hydrolysis of a-hydroxytriazolam-glucoronide in human urine.
Tsujikawa, K., et al.
Health Laboratory Science, 40, 286-289 (2004)
Enzymes
Hans Ulrich Bergmeyer
Methods of Enzymatic Analysis, 460-461 (1974)
V Graef et al.
Clinical chemistry, 23(3), 532-535 (1977-03-01)
We determined the enzymic activity of beta-glucuronidase preparations from bovine liver, Helix pomatia, and Escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. We also studied the effect of Na2SO4 on the enzymic hydrolysis of several substrates with the



국제 무역 품목 번호

SKUGTIN
G7646-25KU04061833643037
G7646-5KU04061833643051
G7646-100KU04061833643020
G7646-500KU04061833643044