G1N350
GenElute™ Mammalian Genomic DNA Miniprep Kits
sufficient for 350 purifications
동의어(들):
Mammalian Genomic DNA Miniprep, animal gDNA prep, gDNA miniprep, mammalian gDNA extraction, mammalian gDNA isolation, mammalian gDNA prep, mammalian gDNA purification, silica mammalian gDNA prep, Gen Elute
크기 선택
제품정보 (DICE 배송 시 비용 별도)
usage
sufficient for 350 purifications
Quality Level
technique(s)
DNA purification: suitable
input
mammalian cells
mammalian tissue
test parameters
sample volume: 25 mg tissue, sample volume: 2x10^6 cells
storage temp.
15-25°C
General description
The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 μL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
The purified genomic DNA is ready for immediate use in downstream applications such as restriction digest, PCR, southern blots, and sequencing reactions.
Application
- to extract DNA from fin samples
- to extract the BSTUI restriction enzyme from the DNA
- to isolate DNA from the blood samples extracted from caudal fin
- to extract tissue and hair follicle DNA
- restriction endonuclease digestions
- PCR
- Southern blots
- sequencing reactions
- cloning
- ligation
Biochem/physiol Actions
Features and Benefits
- Expected yield: 25 μg from 2 x 106 cultured cells; 30 μg from 25 mg of tissue
- Elution volume: 200 - 400 μl
- Time required: 20 min after lysis
- A260/A280 ratio: 1.6 - 1.9
- Mechanical homogenization required: No
Other Notes
Legal Information
signalword
Danger
target_organs
Respiratory system
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
저장 등급
10 - Combustible liquids
프로토콜
The GenElute™ Blood Genomic DNA Kit Protocol provides a simple and convenient way to isolate pure genomic DNA from fresh or aged whole blood.
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
관련 콘텐츠
GenElute™ Mammalian Genomic DNA Miniprep Kit
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| G1N350-1KT | 04061835573042 |