General description
Sigma′s Lambda DNA EcoR I Hind III Digest contains 13 fragments from 125 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
Lambda DNA EcoR I Hind III Digest has been used as DNA markers for comparing the molecular weights of polymerase chain reaction (PCR) amplified products.
Preparation Note
Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.
Other Notes
Contains 13 fragments, 125–21,226 bp
For optimal resolution, the recommended agarose gel concentration is 0.7%.
Sigma′s Lambda DNA EcoR I Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
저장 등급
13 - Non Combustible Solids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
프로토콜
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
문서
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
MOLECULAR CHARACTERIZATION OF FIVE SELECTED BRINJAL (Solanum melongena L) GENOTYPES USING SSR MARKERS
Khorsheduzzaman A K M, et al.
Bangladesh Journal of Plant Breeding and Genetics, 21(1), 01-06 (2008)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| D9281-.1MG | 04061833590560 |
| D9281-.5MG | 04061833590577 |