D8062
DMEM/F-12
With ʟ-glutamine, sodium bicarbonate and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture
동의어(들):
DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture
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제품정보 (DICE 배송 시 비용 별도)
제품 이름
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture
Quality Level
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L
shipped in
ambient
storage temp.
2-8°C
General description
Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.
Application
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:
- to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites
- to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment
- to culture mouse squamous cell carcinoma (SCCVII) tumor cells
저장 등급
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
문서
Calcium is an ionically stable divalent cation with important beneficial and toxic properties in cell culture. It is a component of a wide range of cell culture media.
Nutrient Mixture F-12 Ham Formulation
A new macrolide isolated from the endophytic fungus Colletotrichum sp
Bungihan ME, et al.
3rd Kuala Lumpur International Conference on Biomedical Engineering 2006: Biomed 2006, 6(1), 57-73 (2013)
Hayato Fukusumi et al.
PeerJ, 6, e4187-e4187 (2018-01-10)
Since the development of human-induced pluripotent stem cells (hiPSCs), various types of hiPSC-derived cells have been established for regenerative medicine and drug development. Neural stem/progenitor cells (NSPCs) derived from hiPSCs (hiPSC-NSPCs) have shown benefits for regenerative therapy of the central
Toru Doi et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(43), 10290-10309 (2017-09-22)
Oligodendrocyte precursor cells (OPCs) act as a reservoir of new oligodendrocytes (OLs) in homeostatic and pathological conditions. OPCs are activated in response to injury to generate myelinating OLs, but the underlying mechanisms remain poorly understood. Here, we show that chromodomain
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| D8062-6X500ML | 04061833589182 |
| D8062-500ML | 04061833589175 |