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Merck

D6421

Sigma-Aldrich

DMEM/F-12

With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

동의어(들):

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

MDL number:
MFCD00217417
UNSPSC 코드:
12352207
NACRES:
NA.75

주요 문서

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제품 이름

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

무균

sterile-filtered

양식

liquid

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

성분

sodium pyruvate: 0.055 g/L
L-glutamine: no
phenol red: yes
NaHCO3: yes
HEPES: 15 mM
glucose: 3.15 g/L

배송 상태

ambient

저장 온도

2-8°C

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관련 카테고리

일반 설명

A measured mixture of chemically defined ingredients makes up a basal medium, which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/F-12 is a completely defined synthetic medium, which is protein-free.

애플리케이션

DMEM:F12 is a 50:50 mixture of DMEM and Ham′s F12 media that has proven to be useful in a wide range of cell culture applications, especially when supplemented with fetal bovine serum (FBS). Ham′s Nutrient Mixture F12 was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells, and mouse L cells. It is frequently used with dialyzed serum, hormones, selenium, and other supplements for serum-free cultures. It is the medium of choice for supporting the growth of cells of rodent origin, particularly rabbit and rat, and has been proven to be an excellent cloning medium for myeloma and hybridoma cells.
Suitable for growth of wide range of cell types in low serum conditions.
Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham is used in cell culture & proliferation assays. It is also used in bone marrow-derived macrophages culture.

제조 메모

Supplement with 0.365 g/L L-glutamine.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
RNBL1932
RNBL0503
RNBK9923
RNBK4890
RNBK3728

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