Quality Level
form
liquid
usage
sufficient for 50 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s PCR 50 - 2,000 bp Marker contains 8 dsDNA fragments from 50 – 2,000 bases, evenly spaced. Five μl of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.
Application
PCR 50 - 2,000 bp Marker has been used as a marker for DNA gel electrophoresis.
Other Notes
Contains 8 bands, 50–2,000 bp in predictably spaced (ladder) double-stranded DNA fragments.
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s PCR 50 - 2,000 bp Marker is provided as two tubes:
- 1 vial (250 μl volume) PCR Marker
- 1 vial 6× loading buffer (250 μl volume) for diluting samples
저장 등급
10 - Combustible liquids
ppe
Eyeshields, Gloves
프로토콜
The Extract-N-Amp™ Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
문서
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
관련 콘텐츠
Undergraduate virology exercises demonstrate conventional and real-time PCR using commercially available HIV primers and noninfectious target.
Sulzinski MA, et al.
Biochemistry and Molecular Biology Education, 37, 232-235 (2009)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| P9577-1VL | 04061835573882 |