biological source
fermentation/recombinant (E. coli strain DH5A)
Quality Level
grade
Molecular Biology
form
liquid
usage
80 uses
solubility
water: soluble at 20 °C
suitability
suitable for electrophoresis (DNA)
storage temp.
2-8°C
General description
Sigma′s PCR 20 bp Low Ladder contains 50 dsDNA fragments from 50 – 1,000 bases, evenly spaced in 20 bp repeats. Five μl of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
Application
PCR 20 bp Low Ladder has been used as a marker for DNA gel electrophoresis.
Other Notes
Contains 50 bands, 20–1,000 bp in exact 20 bp repeats.
For optimal resolution, the recommended agarose gel concentration is 2.5%.
Sigma′s PCR 20 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
문서
Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.
Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.
Primary mediastinal B-cell lymphoma: detection of BCL2 gene rearrangements by PCR analysis and FISH.
Dunphy CH, et al.
Journal of Hematopathology, 1, 77-84 (2008)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| P1598-40UG | 04061835573783 |