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P1473

PCR 100 bp Low Ladder

for electrophoresis of PCR fragments

동의어(들):

100bp maker, 100bp marker for agarose gel electrophoresis, 100pb ladder for agarose gel electrophoresis, DNA ladder, DNA marker, gel ladder, gel marker

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크기 선택

보기 변경

제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.25
UNSPSC Code:
41141827
Biological source:
synthetic
Form:
liquid
Storage temp.:
−20°C
기술 서비스
도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.
도움 문의

biological source

synthetic

Quality Level

200

grade

Molecular Biology

form

liquid

usage

75 uses

suitability

suitable for electrophoresis (DNA)

storage temp.

−20°C

General description

Sigma′s PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 – 1,000 bases, evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
The PCR 100 bp low ladder has been specially designed for the size determination of polymerase chain reaction (PCR) generated DNA fragments.

Application

PCR 100 bp Low Ladder has been used:
  • in agarose gel electrophoresis as a standard DNA marker to obtain DNA fragments of about 240 bp for polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) analysis
  • to visualize DNA from enzyme digested PCR mixture
  • to estimate the molecular weight of the PCR product fragments

Other Notes

Contains 10 bands, 100–1,000 bp in exact 100 bp spaced (ladder) recombinant repeats.
For optimal resolution, the recommended agarose gel concentration is 2.0%.
Sigma′s PCR 100 bp Low Marker is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

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시험 성적서(COA)

Lot/Batch Number
088K1503
058K1113
018K1613
087K1146
027K2181

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문서 라이브러리 방문

문서

Selecting DNA, RNA, and PCR Fragment Markers and Ladders for Gel Electrophoresis

Choose the appropriate markers and ladders for nucleic acid size determination of samples separated by electrophoresis. Determine size of DNA, RNA and PCR-generated fragments using agarose or polyacrylamide gels.

Gel Electrophoresis Markers

Markers for gel electrophoresis aid size determination of DNA, PCR fragments, and RNA, staining well with common nucleic acid stains.

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Data Sheet - P1473
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mRNA Synthesis

Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.

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국제 무역 품목 번호

SKUGTIN
P1473-1VL04061835516230