주요 문서

모든 문서 보기

D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

Name: Deoxyribonuclease I RNase-free solution from bovine pancreas
Brand: SIGMA

동의어(들):

DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

보기 변경

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:

9003-98-9

UNSPSC Code:

12352204

NACRES:

NA.53

EC Number:

232-667-0

MDL number:

MFCD00130918

EC 번호:

3.1.21.1 (BRENDA, IUBMB)

Specific activity:

10000 units/mg protein

Biological source:

bovine pancreas

Concentration:

5—15 mg protein/mL

기술 서비스

도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.

도움 문의

속성

biological source

bovine pancreas

Quality Level

200

grade

Molecular Biology

form

buffered aqueous glycerol solution

specific activity

10000 units/mg protein

mol wt

29.1 kDa

concentration

5—15 mg protein/mL

technique(s)

DNA extraction: suitable

suitability

suitable for molecular biology

UniProt accession no.

P00639

application(s)

cell analysis

foreign activity

RNase, none detected

storage temp.

−20°C

Gene Information

cow ... DNASE1(282217)

설명

General description

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg²⁺, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn²⁺, both strands can be cleaved.

Application

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.

Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.

Other Notes

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.

One unit will cause a ΔA₂₆₀ of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.

RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.