R4642
Ribonuclease A from bovine pancreas
(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
동의어(들):
Pancreatic Ribonuclease, RNAsea, RNase A, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
NACRES:
NA.53
UNSPSC Code:
12352204
MDL number:
Biological source:
bovine pancreas
Concentration:
20-40 mg/mL
biological source
bovine pancreas
Quality Level
grade
Molecular Biology
form
(Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)
mol wt
13.7 kDa, ~13,700
concentration
20-40 mg/mL
suitability
suitable for
foreign activity
Endonuclease and exonuclease, none detected, NICKase and DNase, none detected
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
General description
RNase A is an endoribonuclease that attacks at the 3′OHphosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA.
RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.
Application
- RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples.
- RNase A is also used in RNA sequence analysis and protection assays.
- RNase A has been used as a tool for computer-aided drug design.
- RNase A supports the analysis of RNA sequences.
- RNase A hydrolyze RNA contained in protein samples.
- Purification of DNA is supported by RNase A.
Suitable for:
- RNase protection assays
- Removal of unspecifically bound RNA
- Analysis of RNA sequences
- Hydrolysis of RNA contained in protein samples
- Plasmid DNA purification
Features and Benefits
Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.
Analysis Note
Protein determined by E.
Other Notes
A major application for RNase A is the removal of RNA from preparations of plasmid DNA. For this application, DNase free RNase A is used at a final concentration of 10 ug/mL.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Boiling stock solutions of this RNase A product to inactivate residual DNase is not necessary and may cause precipitation of RNase and possible loss of enzymatic activity. If an RNase A solution is heated at a neutral pH, precipitation will occur. When heated at a lower pH, some precipitation may occur because of protein impurities that are present.
Activators of RNase A include potassium and sodium salts. RNase A can be inhibited by alkylation of His12 or His119.
RNase A is supplied as a solution of 50% glycerol containing 10 mM Tris-HCl (pH 8.0).
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
저장 등급
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
프로토콜
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
This procedure may be used for determination of Ribonuclease A (RNase A) activity.
문서
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
사용 가능한 형광 동소 교잡법(FISH) 절차, 시약, 장비.
관련 콘텐츠
Instructions
Wentao Li et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(26), 6752-6757 (2017-06-14)
Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon, is the major cause of lung cancer. BaP forms covalent DNA adducts after metabolic activation and induces mutations. We have developed a method for capturing oligonucleotides carrying bulky base adducts, including UV-induced cyclobutane pyrimidine
Christopher P Selby et al.
The Journal of biological chemistry, 295(50), 17374-17380 (2020-10-23)
In nucleotide excision repair, bulky DNA lesions such as UV-induced cyclobutane pyrimidine dimers are removed from the genome by concerted dual incisions bracketing the lesion, followed by gap filling and ligation. So far, two dual-incision patterns have been discovered: the
Alessandro Ori et al.
Genome biology, 17, 47-47 (2016-03-16)
Recent large-scale studies revealed cell-type specific proteomes. However, protein complexes, the basic functional modules of a cell, have been so far mostly considered as static entities with well-defined structures. The co-expression of their members has not been systematically charted at
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| R4642-10MG | 04061835518760 |
| R4642-1G | 04061835507160 |
| R4642-250MG | 04061835513987 |
| R4642-50MG | 04061835574773 |