P1379
TWEEN® 20
viscous liquid
동의어(들):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
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제품정보 (DICE 배송 시 비용 별도)
Quality Level
description
non-ionic
assay
>40% (GC)
form
viscous liquid
mol wt
~1228
composition
lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)
concentration
≥40.0% (GC)
technique(s)
DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable
impurities
≤3.0% water
refractive index
n20/D 1.468 (lit.)
CMC
0.06 mM (20-25°C)
acid number
≤2.2 mg/g
hydroxyl value
96‑108 mg/g
transition temp
cloud point 76 °C
density
1.095 g/mL at 25 °C (lit.)
HLB
16.7
suitability
complies for IR spectroscopy
application(s)
advanced drug delivery
SMILES string
CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChI key
HMFKFHLTUCJZJO-UHFFFAOYSA-N
General description
Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Tween® 20 is a nonionic surfactant and detergent widely applied in biochemical and life science research. Its predominant use involves the solubilization of membrane proteins during the isolation of membrane-protein complexes. Moreover, it serves as a component in various washing, blocking, dilution, and stabilization buffers. Additionally, it functions as a reagent for immunohistochemistry, encompassing techniques like Western blotting and ELISAs. In these applications, Tween 20 plays crucial roles such as preventing non-specific antibody binding, minimizing background staining, and ensuring the even distribution of reagents.
Surfactants, possessing both hydrophilic and hydrophobic components, fall into two main categories: ionic, which ionize in water (anionic, cationic, or ampholytic), and nonionic, which do not ionize. The discussed product is a nonionic surfactant with diverse applications such as emulsification, dispersion, solubilization, and detergent use. Additionally, it can serve as a solubilizer for membrane proteins.
Surfactants, possessing both hydrophilic and hydrophobic components, fall into two main categories: ionic, which ionize in water (anionic, cationic, or ampholytic), and nonionic, which do not ionize. The discussed product is a nonionic surfactant with diverse applications such as emulsification, dispersion, solubilization, and detergent use. Additionally, it can serve as a solubilizer for membrane proteins.
Application
TWEEN 20 has been used as a component of:
- washing buffer and blocking buffer in Western blotting
- blocking buffer in immunohistochemistry
- washing buffer in ELISA (enzyme linked immunosorbent assay)
- reaction mixture in PCR (polymerase chain reaction)
Non-ionic detergent
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
Features and Benefits
- Highly versatile surfactant for your life science and biochemical research
- Excellent solubilising, dispersing and emulsifying agent
- Suitable for DNA & RNA extraction, mammalian cell culture, and protein purification
Packaging
P1379-1KT:
Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.
Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.
Preparation Note
TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
TWEEN is a registered trademark of Croda International PLC
저장 등급
10 - Combustible liquids
flash_point_f
527.0 °F - Pensky-Martens closed cup
flash_point_c
275 °C - Pensky-Martens closed cup
ppe
Eyeshields, Gloves
프로토콜
This page shows how to solubilize membrane proteins with products from Cytiva.
문서
Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.
사용 가능한 형광 동소 교잡법(FISH) 절차, 시약, 장비.
Gene expression and immune response kinetics using electroporation-mediated DNA delivery to muscle.
Gr?nevik E et al
The journal of gene medicine, 7(2), 218-227 (2005)
Use of tRNA consensus primers to indicate subgroups of Pseudomonas solanacearum by polymerase chain reaction amplification.
Seal SE et al
Applied and Environmental Microbiology, 58(11), 3759-3761 (1992)
Polysorbates 20 and 80 used in the formulation of protein biotherapeutics: structure and degradation pathways.
Kerwin BA
J. Pharm. Pharm. Sci., 97(8), 2924-2935 (2008)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| P1379-100ML | 04061837602108 |
| P1379-1KT | 04061842429400 |
| P1379-4L | 04061834354611 |
| P1379-1L | 04061837602115 |
| P1379-250ML | 04061838672940 |
| P1379-25ML | 04061837602139 |
| P1379-500ML | 04061837602153 |
| P1379-6X1L | 04061837602160 |
| P1379-6X500ML | 04061837602177 |