93773
TWEEN® 20
viscosity 250-450 mPa.s (25 °C)
동의어(들):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
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제품정보 (DICE 배송 시 비용 별도)
description
non-ionic
Quality Level
form
viscous liquid
mol wt
~1228
technique(s)
DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable
refractive index
n20/D 1.468 (lit.), n20/D 1.471
viscosity
250-450 mPa.s(25 °C)
HLB value
16.7±1.0
density
1.11 g/mL at 20 °C, 1.095 g/mL at 25 °C (lit.)
SMILES string
CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO
InChI
1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3
InChI key
HMFKFHLTUCJZJO-UHFFFAOYSA-N
General description
Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Application
Non-ionic detergent
Tween® 20 is widely used in several biochemical applications. It has been used:
- as an emulsifying agent for the preparation of stable oil-in-water emulsions
- in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
- as a blocking agent for membrane based immunoassays at a typical concentration of 0.05% - 1%
- for lysing mammalian cells at a concentration of 0.005 to 0.5%
- for rehydrating tissue before in situ hybridization
Biochem/physiol Actions
Tween® 20, functioning as a detergent, potentially operates by binding to the hydrophobic components of water-insoluble substances, thereby making them hydrophilic.
Features and Benefits
- Suitable for protein purification and DNA and RNA extraction
- Suitable for mammalian cell culture
- High purity product for research applications
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
TWEEN is a registered trademark of Croda International PLC
Disclaimer
may get turbid on storage, heat to clear
저장 등급
10 - Combustible liquids
flash_point_f
527.0 °F - Pensky-Martens closed cup
flash_point_c
275 °C - Pensky-Martens closed cup
ppe
Eyeshields, Gloves
The role of formyl peptide receptor 1 (FPR1) in neuroblastoma tumorigenesis.
Snapkov I, et al.
BMC Cancer, 16(1), 490-490 (2016)
Temporal expression of cytokines and signal transducer and activator of transcription factor 3 activation after neonatal hypoxia/ischemia in mice.
Snapkov I, et al.
Developmental Neuroscience, 35(2-3), 212-225 (2013)
Reynolds JEF
Martindale: The Extra Pharmacopoeia, 1030-1030 (1993)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 93773-1KG | 04061833283196 |
| 93773-250G | 04061833283202 |