Quality Level
technique(s)
whole genome amplification: suitable
dilution
(WTA)
input
purified RNA
shipped in
wet ice
storage temp.
−20°C
General description
The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.
Application
SeqPlex™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.
Features and Benefits
- Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
- Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
- No need to fragment DNA before sequencing.
- Amplifies ds-cDNA in 8 hours or less.
- Compatible with all next generation sequencing platforms except Pacific Bioscience.
Other Notes
SEQR-500RXN is manufactured on-demand. Contact us for more information.
Legal Information
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
저장 등급
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
프로토콜
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
DNA Sequencing Research Group (DSRG): Evaluation of RNA Amplification Kits at Subnanogram Input Amounts of Total RNA for RNA-Seq
Nicolet C, et al.
Journal of biomolecular techniques : JBT, 24, S70-S70 (2013)
Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
Grégory Seumois et al.
Nature immunology, 15(8), 777-788 (2014-07-07)
A characteristic feature of asthma is the aberrant accumulation, differentiation or function of memory CD4(+) T cells that produce type 2 cytokines (TH2 cells). By mapping genome-wide histone modification profiles for subsets of T cells isolated from peripheral blood of
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| SEQR-500RXN | 04061837018114 |
| SEQR-10RXN | 04061837018107 |
| SEQR-50RXN | 04061837018121 |