콘텐츠로 건너뛰기
Merck

주요 문서

모든 문서 보기

S9430

SYBR® Green I nucleic acid gel stain

greener alternative

10,000 × in DMSO

동의어(들):

DNA stain, SYBR® green gel dye, safer gel stain

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

보기 변경

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
163795-75-3
UNSPSC Code:
12171500
NACRES:
NA.52
MDL number:
MFCD00284715
기술 서비스
도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.
도움 문의

form

solution

Quality Level

200

usage

 mL sufficient for 100 mini-gels

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

concentration

10,000 × in DMSO

technique(s)

PCR: suitable

greener alternative category

Aligned,

storage temp.

−20°C

General description

SYBR® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.

Application

SYBR® Green I nucleic acid gel stain has been used for:
  • the quantification of dsDNA
  • to stain DNA in polymerase chain reaction (PCR)
  • for comet assay technique
  • to assess spermatozoon membrane integrity
  • for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)
  • as a fluorescent dye in flow cytometry
  • real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA

Features and Benefits

  • An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
  • It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
  • It is less mutagenic than ethidium bromide in Ames tests
  • It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
  • Useful for many applications with a limited amount of DNA
  • The binding of SYBR® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
  • Removal of this stain in-gel digestion and ligation techniques is not needed
  • SYBR Green I is a greener alternative product to ethidium bromide for staining

Preparation Note

Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.

Legal Information

SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

저장 등급

10 - Combustible liquids

wgk

WGK 3

flash_point_f

201.2 °F - closed cup

flash_point_c

94 °C - closed cup

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
048K1041
127K1616
057K1386
057K1363
037K1047

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

프로토콜

SeqPlex DNA Amplification Kit Protocol

SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.

Hot Start dNTP Protocol

Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

SEQR - SeqPlex RNA Amplification Kit Protocol

The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.

모든 프로토콜 보기

문서

SeqPlex™-I Transcriptome Amplification Kits

SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.

Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications
Zipper H, et al.
Nucleic Acids Research, 32(12), e103-e103 (2004)
P A Morin et al.
BioTechniques, 19(2), 223-228 (1995-08-01)
A method for resolving and visualizing genotypes for simple sequence repeat loci on short (20 cm) polyacrylamide gels is described. This method makes use of a modified vertical electrophoresis cell to allow rapid electrophoresis without variation in migration rates due
Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering
Tresoldi C, et al.
Materials Science and Engineering, C, 105, 110035-110035 (2019)
모든 관련된 서류 보기

국제 무역 품목 번호

SKUGTIN
S9430-.5ML04061837899140
S9430-1ML04061837899164