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WTA2

Complete Whole Transcriptome Amplification Kit

DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias

동의어(들):

transcriptome amplification kit

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제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.55
UNSPSC Code:
41121800
기술 서비스
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도움 문의

Quality Level

200

technique(s)

whole genome amplification: suitable

dilution

(WTA)

input

purified RNA

shipped in

wet ice

storage temp.

−20°C

General description

WTA2 is optimized to amplify RNA from formalin-fixed, paraffin-embedded (FFPE) and other damaged or degraded samples. Whole Transcriptome Amplification (WTA) technology, allows for representative amplification of low nanogram quantities of total RNA in less than 4 hours without 3′-bias. Amplification products are suitable for applications such as qPCR, micro array analysis, and cloning. The WTA2 kit contains the polymerase needed to amplify the cDNA library.

Application

Complete Whole Transcriptome Amplification Kit is used for the following applications:
  • To establish a protocol for the simultaneous analysis of DNA and RNA viruses present in pig feces using process controlled deep sequencing.
  • Reverse transcription and cDNA amplification
  • For the synthesis and amplification of cDNA library using Genomic RNA released from immunocaptured PPV particles
  • Nucleic Acid Preparation and Deep Sequencing (The extracted nucleic acids were randomly primed for cDNA synthesis)
  • Amplification of RNA andssDNA viruses for Viral metagenomics from low-input clinical samples
Suitable for use with downstream applications including:
  • qPCR
  • microarray analysis
  • cloning

Biochem/physiol Actions

The WTA2 process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. During this process, displaced single strands serve as new templates for primer annealing and extension. The resultant cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence. The 2nd step amplifies the cDNA library by PCR using WTA2 polymerase and a universal end primer to produce WTA2 product.

Features and Benefits

  • Achieve up to 10,000x amplification in less than 4 hours with less than 30 minutes of "hands on" time required
  • Only 20 pg of total RNA template is required to amplify suitable cDNA for microarray profiling
  • Contains all needed components for cDNA amplification
  • Achieve linear amplification of expressed genes and exons without 3′ or 5′ bias
  • Effectively amplifies single cell or low input RNA, including mRNA and total RNA from any animal, plant, or microorganism

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS & 가격
  • Library Synthesis Enzyme
    SDS
  • Library Synthesis Solution
    SDS
  • Amplification Mix
    SDS
  • Library Synthesis Buffer
    SDS
  • W4502
    Water, Nuclease-Free Water, for Molecular Biology
    SDS
  • Amplification Enzyme
    SDS
  • D7295
    Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent
    SDS

저장 등급

10 - Combustible liquids

wgk

WGK 3

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시험 성적서(COA)

Lot/Batch Number
098K6134
098K6102
098K6097
088K6199

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문서 라이브러리 방문

프로토콜

Integration of Sigma® TransPlex® with Agilent Microarray

TransPlex® kits' amplification products integrate seamlessly into existing Agilent workflows for microarray target expression analyses.

Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

문서

WTA2 Amplicon Modification for NGS

Transplex Whole Transcriptome Amplification (WTA2) precisely amplifies RNA maintaining transcript levels in test and reference samples.

Whole Transcriptome Amplification of RNA from Low Cell-Number Samples

The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.

관련 콘텐츠

Product Information Sheet - WTA2
Whole Transcriptome Amplification FAQs

Transplex Whole Transcriptome Amplification FAQs on topics including whole transcriptome steps, RNA source, including archival fixed tissue, library purification, quantitation of the product and downstream applications

Bert Vanmechelen et al.
Scientific reports, 8(1), 11171-11171 (2018-07-26)
The family Arteriviridae harbors a rapidly expanding group of viruses known to infect a divergent group of mammals, including horses, pigs, possums, primates, and rodents. Hosts infected with arteriviruses present with a wide variety of (sub) clinical symptoms, depending on
Bert Vanmechelen et al.
BMC genomics, 19(1), 617-617 (2018-08-18)
In the past decade, many new paramyxoviruses that do not belong to any of the seven established genera in the family Paramyxoviridae have been discovered. Amongst them are J-virus (JPV), Beilong virus (BeiPV) and Tailam virus (TlmPV), three paramyxovirus species
Jana Sachsenröder et al.
PloS one, 9(2), e88888-e88888 (2014-03-04)
The pig faecal virome, which comprises the community of viruses present in pig faeces, is complex and consists of pig viruses, bacteriophages, transiently passaged plant viruses and other minor virus species. Only little is known about factors influencing its general
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국제 무역 품목 번호

SKUGTIN
WTA2-10RXN04061835378814
WTA2-50RXN04061835378821