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C7594

Casein Blocking Buffer

for Northern and Southern blotting, powder blend

Synonym(s):

blocking buffer

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About This Item

EC Number:
232-555-1
UNSPSC Code:
12161700
NACRES:
NA.25
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grade

Molecular Biology

Quality Level

200

form

powder blend

technique(s)

Southern blotting: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

storage temp.

room temp

General description

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

Application

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

Features and Benefits

  • Better blocking performance than dried milk or BSA
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays

Preparation Note

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

Storage Class

11 - Combustible Solids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
088K8211
117K8204
037K8209
056K8212
038H8210

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Casein Blocking Buffer Protocol

Protocol for Casein Blocking Buffer in Southern and Western blotting includes troubleshooting for optimizing signal.

Northern und Southern Blot Protokolle und Einführung

Einführung in Northern Blotting und Southern Blotting, bei denen es sich um beliebte Methoden zur Übertragung von Makromolekülen auf Membranen handelt. Dieser Artikel enthält außerdem ein Protokoll für Northern Blotting und ein Protokoll für Southern Blotting.

Related Content

Data Sheet - C7594

Instructions

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Global Trade Item Number

SKUGTIN
C7594-1L04061833513040