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D2532

Denhardt′s Solution 50x

Name: Denhardt′s Solution 50x
Brand: SIGMA

for Northern and Southern blotting, solution

Synonym(s):

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

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About This Item

NACRES:

NA.25

UNSPSC Code:

12161700

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Properties

Quality Level

300

form

solution

technique(s)

Southern blotting: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

−20°C

Description

General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Preparation Note

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Ficoll^® 400 for Gradient Centrifugation

Ficoll 400^® is a non-ionic polymer with high hydrophilicity and water solubility, suitable for various biological applications.

Ficoll^® 400 für die Gradientenzentrifugation

Ficoll^®-400 ist ein hochverzweigtes Polymer, das durch Copolymerisation von Saccharose und Epichlorhydrin gebildet wird. Ficoll® 400 ist vollständig nicht-ionisch. Aufgrund der großen Anzahl an Hydroxylgruppen ist Ficoll® 400 sehr hydrophil und extrem wasserlöslich.

View All Protocols

Global Trade Item Number

SKU	GTIN
D2532-5X5ML	04061838255143
D2532-5ML	04061838104885