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93017

SSC Buffer 20x

Name: SSC Buffer 20x
Brand: SIGMA

for Northern and Southern blotting, solution

Synonym(s):

20x SSC

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About This Item

NACRES:

NA.25

UNSPSC Code:

12161700

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Properties

grade

Molecular Biology

Quality Level

200

product line

BioReagent

form

solution

technique(s)

Southern blotting: suitable

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

pH

7.0±0.2

density

1.16 g/mL at 20 °C

storage temp.

room temp

Description

General description

SSC Buffer 20x Concentrate is a standard reagent in Southern and Northern blotting procedures. This concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Application

Suitable for:

Preparing nucleic acids for hybridization
Hybridization buffer for Northern and Southern blots
Washing buffer for Northern and Southern blots

Features and Benefits

Convenient ready-to-use liquid
Multi-purpose use in hybridizations
Easily diluted to any concentration needed

Preparation Note

SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Protocols

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Northern und Southern Blot Protokolle und Einführung

Einführung in Northern Blotting und Southern Blotting, bei denen es sich um beliebte Methoden zur Übertragung von Makromolekülen auf Membranen handelt. Dieser Artikel enthält außerdem ein Protokoll für Northern Blotting und ein Protokoll für Southern Blotting.

Articles

Water for Nucleic Acid Gel Electrophoresis

Learn how water impurities can affect DNA and RNA electrophoresis and blotting. High purity water purified using ultrafiltration is suitable for nucleic acid electrophoresis, Northern and Southern blotting.

Integration-deficient lentivectors: an effective strategy to purify and differentiate human embryonic stem cell-derived hepatic progenitors.

Guanghua Yang et al.

BMC biology, 11, 86-86 (2013-07-23)

Human pluripotent stem cells (hPSCs) hold great promise for applications in regenerative medicine. However, the safety of cell therapy using differentiated hPSC derivatives must be improved through methods that will permit the transplantation of homogenous populations of a specific cell

Expression profiling in vivo demonstrates rapid changes in lung microRNA levels following lipopolysaccharide-induced inflammation but not in the anti-inflammatory action of glucocorticoids.

Sterghios A Moschos et al.

BMC genomics, 8, 240-240 (2007-07-21)

At present, nothing is known of the role of miRNAs in the immune response in vivo despite the fact that inflammation is thought to underlie multiple acute and chronic diseases. In these circumstances, patients are commonly treated with corticosteroids such

Global Trade Item Number

SKU	GTIN
93017-10L-F	04061833271360