CHP1
Imprint® Chromatin Immunopreciptitation Kit
Complete ChIP reaction in 6 hours in flexible strip well format
Synonyme(s) :
Chromatin Immunoprecipitation
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A propos de cet article
NACRES:
NA.54
UNSPSC Code:
41116158
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storage temp.
−20°C
General description
Imprint Chromatin Immunoprecipitation Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification and an integrated protocol for ChIP DNA amplification with our GenomePlex® Whole Genome Amplification Kit (WGA2) . The flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or tissue in a convenient strip-well format.
For Frequently Asked Questions about this kit, please see ChIP Troubleshooting Questions.
For Frequently Asked Questions about this kit, please see ChIP Troubleshooting Questions.
Application
The Imprint Chromatin Immunoprecipitation Kit is a flexible, rapid and complete method for investigating protein-DNA interactions by chromatin immunoprecipitation (ChIP).
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Features and Benefits
- Fast—Total protocol time of less than 6 hours making the Imprint kit the fastest on the market
- Sensitive—As few as 10,000 cells required for each ChIP sample
- Convenient—Fewest steps of any available ChIP protocol
- Flexible—Protocols for cells or tissue, and convenient strip-well format for high-throughput applications
- Complete—Includes columns and reagents for DNA purification as well as an integrated protocol for amplification with the GenomePlex technology
Legal Information
GenomePlex is a registered trademark of Takara Bio USA, Inc.
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Composants de kit également disponibles séparément
Réf. du produit
Description
FDS & Tarif
- Proteinase K from Tritirachium album, buffered aqueous glycerol solution, Molecular Biology, ≥800 units/mL
signalword
Danger
target_organs
Central nervous system
Classe de stockage
3 - Flammable liquids
flash_point_f
53.6 °F - closed cup
flash_point_c
12 °C - closed cup
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
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Protocoles
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
Contenu apparenté
Ghazala Begum et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(4), 1694-1703 (2012-01-10)
Undernutrition during pregnancy is implicated in the programming of offspring for the development of obesity and diabetes. We hypothesized that maternal programming causes epigenetic changes in fetal hypothalamic pathways regulating metabolism. This study used sheep to examine the effect of
Poulomi Banerjee et al.
Journal of molecular medicine (Berlin, Germany), 94(12), 1397-1409 (2016-08-19)
Epithelial-mesenchymal transition (EMT) is one of the key biological phenomena behind cancer and metastasis. Clinical studies suggest that patients undergoing metformin therapy are less predisposed to cancer but the underlying mechanism is far from clear. Given that metformin also acts
Lucie Hyrsova et al.
British journal of pharmacology, 173(10), 1703-1715 (2016-02-28)
The organic cation transporter 1 (OCT1) transports cationic drugs into hepatocytes. The high hepatic expression of OCT1 is controlled by the HNF4α and USF transcription factors. Pregnane X receptor (PXR) mediates induction of the principal xenobiotic metabolizing enzymes and transporters
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| APREST71098-100UL | 04061835939237 |
| HPA006616-100UL | 04061835679980 |
| HPA006616-25UL | 04061842777327 |
| CHP1-24RXN | 04061833531815 |
| CHP1-96RXN | 04061833531822 |
| EHU110131-20UG | 04061828593064 |
| EHU110131-50UG | 04061828356270 |