P4978
Phosphatase, Alkaline from calf intestine
buffered aqueous glycerol solution
Synonyme(s) :
CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
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A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
eCl@ss:
42010105
NACRES:
NA.53
MDL number:
Concentration:
≥10,000 units/mL
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Molecular Biology
form
buffered aqueous glycerol solution
mol wt
~80 kDa
concentration
≥10,000 units/mL
UniProt accession no.
foreign activity
DNase, RNase, none detected
shipped in
wet ice
storage temp.
−20°C
Gene Information
cow ... ALPI(280993)
General description
Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.
Application
Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.
Other Notes
Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.
Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.
One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.
Classe de stockage
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocoles
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.
Contenu apparenté
Yusheng Zhao et al.
Genes & development, 35(11-12), 888-898 (2021-05-15)
Plants monitor many aspects of their fluctuating environments to help align their development with seasons. Molecular understanding of how noisy temperature cues are registered has emerged from dissection of vernalization in Arabidopsis, which involves a multiphase cold-dependent silencing of the
J Juhásová et al.
Physiological research, 60(3), 559-571 (2011-03-16)
Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteogenic differentiation of miniature pig MSCs and markers of this differentiation in vitro. Flow-cytometrically characterized MSCs
M Koolen et al.
European cells & materials, 38, 94-105 (2019-09-19)
This study aimed at investigating in vitro and in vivo the efficiency of commercially available fibrin as a carrier for controlled and sustained bone morphogenetic protein-2 (BMP-2) release to induce bone formation and reduce the side effects of its use.
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| P4978-500UN | 04061834375586 |
| P4978-1KU | 04061835574667 |