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D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

Synonyme(s) :

DNase I, Deoxyribonuclease I, pancreatic DNase I, pancreatic deoxyribonuclease I

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A propos de cet article

Numéro CAS:
9003-98-9
UNSPSC Code:
12352204
NACRES:
NA.53
EC Number:
232-667-0
MDL number:
MFCD00130918
Numéro CE :
3.1.21.1 (BRENDA, IUBMB)
Specific activity:
10000 units/mg protein





Biological source:
bovine pancreas
Concentration:
5—15 mg protein/mL
Service technique
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biological source

bovine pancreas

Quality Level

200

grade

Molecular Biology

form

buffered aqueous glycerol solution

specific activity

10000 units/mg protein





mol wt

29.1 kDa

concentration

5—15 mg protein/mL

technique(s)

DNA extraction: suitable

suitability

suitable for molecular biology

UniProt accession no.

P00639

application(s)

cell analysis

foreign activity

RNase, none detected

storage temp.

−20°C

Gene Information

cow ... DNASE1(282217)

General description

Deoxyribonuclease I digests single- and double-stranded DNA to a mixture of mono and oligonucleotides carrying 5′phosphates and 3′OH termini. This catalytic activity is divalent ion-dependent. In the presence of Mg2+, DNase I hydrolyzes each strand of double-stranded DNA randomly and independently. In the presence of Mn2+, both strands can be cleaved.

Application

Used in molecular biology applications for removing DNA during RNA purification, for preparing DNA for nick translation, and for DNA-protein interaction analysis by footprinting methods.
Deoxyribonuclease I RNase-free solution from bovine pancreas has been used to digest DNA from various samples.

Other Notes

DNase I is provided in a solution of 50% (v/v) glycerol, 20 mM sodium acetate (pH 6.5), 5 mM CaCl2, and 0.1 mM PMSF.
One unit will cause a ΔA260 of 0.001 per min per mL reaction mixture using calf thymus DNA as substrate.
RNA treated with this DNase I should not be used to generate a cDNA library or used for RT-PCR. For these sensitive applications, it is recommended to use DNase I, Amplification Grade , Catalog Number AMP-D1.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Classe de stockage

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificats d'analyse (COA)

Lot/Batch Number
108K1173
108K1693
098K1466
088K1433
068K1216

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Protocoles

Deoxyribonuclease I

Deoxyribonuclease I is isolated from bovine pancreas and is processed to reduce RNase activity to below detectable levels.

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Numéro d'article de commerce international

RéférenceGTIN
D7291-.5MG04061833588376
D7291-2MG04061833588383