N1891
p-Nitrophenyl phosphate
SIGMAFAST™, chromogenic, tablet
Synonyme(s) :
pNPP tablets
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
A propos de cet article
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderNom du produit
SIGMAFAST™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 5 mL
Quality Level
form
tablet
solubility
deionized water: soluble 5 mL
storage temp.
−20°C
Application
Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.
SIGMA FAST p-Nitrophenyl phosphate Tablets has been used:
- to quantify non-secreted alkaline phosphatase to monitor differentiation of osteoblast-like cells (SaoS-2)
- to stain murine preosteoblast MC3T3-E1 cells in alkaline phosphatase assay
- as a substrate for ExtrAvidin® alkaline phosphatase to measure hemoglobin-bound α-synuclein using enzyme-linked immunosorbent assay (ELISA)
- as a substrate for alkaline phosphatase conjugated antibodies for detection in ELISA of abirin toxin
Preparation Note
Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing pNPP.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Protocoles
Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.
Articles
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
Zimu Wu et al.
International journal of clinical and experimental pathology, 12(5), 1757-1763 (2020-01-15)
Post-stroke induction of alpha-synuclein (AS), a neuronal protein implicated in the pathogenesis of Parkinson's disease (PD), has been demonstrated to induce secondary brain damage after cerebral ischemia. Therefore, understanding the expression and pathogenic modifications of AS is clinically meaningful for
Conall H Watson et al.
PLoS neglected tropical diseases, 11(7), e0005786-e0005786 (2017-07-21)
Fiji, an upper-middle income state in the Pacific Ocean, has experienced an increase in confirmed case notifications of enteric fever caused by Salmonella enterica serovar Typhi (S. Typhi). To characterize the epidemiology of typhoid exposure, we conducted a cross-sectional sero-epidemiological
Ruklanthi de Alwis et al.
The Journal of infectious diseases, 219(2), 295-304 (2018-10-16)
Nontyphoidal Salmonella (NTS) organisms are a major cause of gastroenteritis and bacteremia, but little is known about maternally acquired immunity and natural exposure in infant populations residing in areas where NTS disease is highly endemic. We recruited 503 pregnant mothers
Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| N1891-50SET | 04061835557943 |
| N1891-5SET | 04061834109907 |