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MilliporeSigma

P8340

Sigma-Aldrich

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use with mammalian cell and tissue extracts, DMSO solution

Synonym(s):

Protease Inhibitor Mix

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About This Item

MDL number:
MFCD00677817
UNSPSC Code:
12352200
NACRES:
NA.77
Form:
DMSO solution
Solubility:
water: soluble
Storage temp.:
−20°C

Key Documents

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Product Name

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

biological source

bovine lung

Quality Level

300

form

DMSO solution

solubility

water: soluble

storage temp.

−20°C

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General description

Protease Inhibitor Cocktail for mammalian cell and tissue extracts, in DMSO solution. It contains six individual components for inhibiting serine, cysteine, and acid proteases, and aminopeptidases.

Application

Ideal for use as a buffer component during sonication, lysis buffer, and RIPA buffer

Provides protection against endogenous enzyme activity in cell and tissue extracts

Biochem/physiol Actions

Inhibits serine, cysteine, and acid proteases, and aminopeptidases.
This mixture contains individual components, including AEBSF at 104 mM, Aprotinin at 80 μM, Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases.

Features and Benefits

Broad-spectrum inhibition: inhibits multiple classes of proteases and aminopeptidases

Stable storage: supplied as a solution in DMSO, allowing for long-term storage at -20°C

Consistent performance: ready-to-use solution

Preparation Note

One mL of this cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g of bovine liver or 10 mL of lysate from CHO cells. This product is supplied as a solution in DMSO.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Disclaimer

This cocktail should be stored at -20 °C.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
109M4069V
109M4068V
11181130
039M4113V
039M4088V

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A transgenic rat that develops Alzheimer's disease-like amyloid pathology, deficits in synaptic plasticity and cognitive impairment.
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Planel E et al
Faseb Journal, 23(8), 2595-2604 (2009)
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Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

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