P5726
Phosphatase Inhibitor Cocktail 2
aqueous solution (dark coloration may develop upon storage, which does not affect the activity), for the inhibition of tyrosine protein phosphatases, acid and alkaline phosphatases
Synonym(s):
Protease inhibitor
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352200
NACRES:
NA.54
Form:
aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
Solubility:
water: soluble
Storage temp.:
2-8°C
Product Name
Phosphatase Inhibitor Cocktail 2, aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
form
aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
Quality Level
solubility
water: soluble
shipped in
wet ice
storage temp.
2-8°C
General description
Phosphatase Inhibitor Cocktail 2 is a mixture of components designed to inhibit enzymes that modify proteins present in cell extracts, leading to improved yields of intact proteins. It has been optimized and tested for use on various animal tissues and cytosolic extracts.
Application
The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been optimized and tested for tyrosine protein phosphatases, acid and alkaline phosphatases. It has been tested on cell extracts from animal tissues including bovine liver and human placenta, and cytosolic extract of rabbit muscle.
Biochem/physiol Actions
This mixture contains individual components with specific inhibitory properties. Sodium orthovanadate inhibits a number of ATPases, protein tyrosine phosphatases, and other phosphate-transferring enzymes. Sodium molybdate inhibits acid and phosphoprotein phosphatases. Sodium tartrate inhibits acid phosphatases. Imidazole inhibits alkaline phosphatases.
Features and Benefits
This compound is a featured product for Kinase Phosphatase Biology research. Click here to discover more featured Kinase Phosphatase Biology products. Learn more about bioactive small molecules for other areas of research at sigma.com/discover-bsm.
Preparation Note
This cocktail is supplied as a clear aqueous solution. It has been sterile filtered through a 0.2 μm membrane in bottles that have been aseptically filled. One mL will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, bovine liver, rabbit muscle, A431, or Jurkat cell extracts at a protein concentration of approximately 5 mg/mL.
Disclaimer
As supplied, this product is stable for two years at the recommended 2-8°C storage temperature.
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Articles
Discover Bioactive Small Molecules for Kinase Phosphatase Biology
The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.
Related Content
Blanca Majem et al.
Oncogene, 38(32), 6035-6050 (2019-07-07)
Ovarian cancer is the most lethal gynecological malignancy due to the silent nature on its early onset and the rapid acquisition of drug resistance. Histologically heterogeneous, it includes several subtypes with different mutational landscapes, hampering the development of effective targeted
Colin S McCoin et al.
American journal of physiology. Endocrinology and metabolism, 317(2), E298-E311 (2019-05-01)
The impact of sexual dimorphism and mitophagy on hepatic mitochondrial adaptations during the treatment of steatosis with physical activity are largely unknown. Here, we tested if deficiencies in liver-specific peroxisome proliferative activated-receptor-γ coactivator-1α (PGC-1α), a transcriptional coactivator of biogenesis, and
Shira Simonovitch et al.
Journal of Alzheimer's disease : JAD, 70(3), 861-875 (2019-07-16)
This study examined the effects of apolipoprotein E4 (APOE4), the most prevalent genetic risk factor for Alzheimer's disease (AD), on proteins involved in mitochondrial dynamics and autophagy, in the hippocampus of targeted replacement mice. Immunohistochemical measurements revealed that the levels
Global Trade Item Number
| SKU | GTIN |
|---|---|
| P5726-300UL | 04061838249678 |
| P5726-1ML | 04061838446503 |
| P5726-5ML | 04061838446510 |