G2N70
GenElute™ Plant Genomic DNA Miniprep Kit
sufficient for 70 purifications
Synonym(s):
Plant Genomic DNA Miniprep, Gen Elute
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About This Item
NACRES:
NA.55
UNSPSC Code:
41105501
usage
sufficient for 70 purifications
Quality Level
greener alternative product characteristics
Inherently Safer Chemistry for Accident Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
greener alternative category
, Aligned
storage temp.
15-25°C
General description
The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
The GenElute Plant Genomic DNA Purification Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of plant species. This kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform.
Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns.
The purified genomic DNA is ready for immediate use in downstream applications such as PCR, restriction digestion, cloning and Southern blots.
Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns.
The purified genomic DNA is ready for immediate use in downstream applications such as PCR, restriction digestion, cloning and Southern blots.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
GenElute™ Plant Genomic DNA Miniprep Kit has been used:
- PCR
- restriction endonuclease digestion
- cloning
- Southern blots
GenElute™ Plant Genomic DNA Miniprep Kit has been used:
- in the purification of genomic DNA isolated from neem tissues
- to extract genomic DNA from embryos
- to isolate DNA from plant samples
The purified genomic DNA is ready for immediate use in sensitive downstream applications such as:
- PCR
- restriction endonuclease digestion
- cloning
- Southern blots
Biochem/physiol Actions
Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length.
Features and Benefits
- Starting material: Up to 100 mg of plant tissue
- Expected yield: Up to 20 μg
- Elution volume: 100 - 200 μl
- Time required: < 40 min
- RNase treatment required: No
Other Notes
For additional information, please see www.sigma-aldrich.com/genomicdna.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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Kit Components Also Available Separately
Product No.
Description
SDS & Pricing
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1C
supp_hazards
Storage Class
8A - Combustible corrosive hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 2
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Protocols
This protocol describes a simple and convenient procedure to isolate pure DNA from a variety of plant species using the GenElute Plant Genomic DNA Miniprep Kit.
Articles
Extract-N-Amp kits extract and amplify genomic DNA, optimized for plant tissue without inhibition concerns.
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
Comparative Analysis of the Genomic DNA Isolation Methods on Inula sp.(Asteraceae)
SEViNDiK E, et al.
Notulae Scientia Biologicae, 8(4), 451-455 (2016)
The optimization of voltage parameter for tissue electroporation in somatic embryos of Astragalus chrysochlorus (Leguminosae)
Turgut-Kara N and Ari S
African Journal of Biotechnology, 9(29), 4584-4588 (2010)
Václav Bačovský et al.
International journal of molecular sciences, 23(5) (2022-03-11)
Young sex chromosomes possess unique and ongoing dynamics that allow us to understand processes that have an impact on their evolution and divergence. The genus Silene includes species with evolutionarily young sex chromosomes, and two species of section Melandrium, namely
Global Trade Item Number
| SKU | GTIN |
|---|---|
| G2N70-1KT | 04061835573073 |