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MilliporeSigma

W4502

Water

suitable for electrophoresis, PCR

Synonym(s):

DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water

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About This Item

Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
NACRES:
NA.31
PubChem Substance ID:
UNSPSC Code:
12191602
EC Number:
231-791-2
MDL number:
Beilstein/REAXYS Number:
2050024
Technique(s):
PCR: suitable, electrophoresis: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
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Product Name

Water, Nuclease-Free Water, for Molecular Biology

vapor density

<1 (vs air)

Quality Level

vapor pressure

3 mmHg

sterility

0.1 μm filtered

form

liquid

technique(s)

PCR: suitable, electrophoresis: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, RNase, Protease, free

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

Application

Water has been used-
  • in cDNA synthesis and PCR amplification
  • for the dilution of rumen DNA sample prior to qPCR assay
  • in PCR (polymerase chain reaction) and agarose gel electrophoresis
  • for the elution of RNA during RNA isolation

Features and Benefits

  • Nuclease-Free Water
  • DNase-Free, RNase-Free, Protease- Free
  • Not DEPC Treated
  • 0.1 um Filtered
  • Prepared from Double Distilled and Deionized Water
  • Suitable for use in Molecular Applications

This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC).

Preparation Note

This product is prepared from double distilled and deionized water

Other Notes

Easily compare specifications for Water products with the Water specification table.


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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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Protocols

Protocol for the preparation of Antibody Sensitized Sheep Erythrocytes. Includes product numbers and links.

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

Whole Genome Amplification products, including kits for DNA extraction, support various DNA sources.

View All Protocols

Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water.
Skraber S et al
Applied and Environmental Microbiology, 70(6), 3644-3649 (2004)
Presence of viral genomes in mineral water: a sufficient condition to assume infectious risk?
Gassilloud B et al
Applied and Environmental Microbiology, 69(7), 3965-3969 (2003)
Polymorphic exact tandem repeat A (PETRA): a newly defined lineage of mycobacterium tuberculosis in israel originating predominantly in Sub-Saharan Africa.
Freidlin PJ et al
Journal of Clinical Microbiology, 47(12), 4006-4020 (2009)



Global Trade Item Number

SKUGTIN
W4502-1.5ML04061837774577
W4502-100ML04061837774591
W4502-5ML04061837774744
W4502-6X1L04061837899300
W4502-1L04061837899294
W4502-10L04061837899287
W4502-10ML04061837774614
W4502-10X50ML04061841755142
W4502-1ML04061837774645
W4502-25ML04061837774683
W4502-50ML04061837774720
W4502-6X1L-I04061837899317