Anti-β-Tubulin III Antibody

50 kDa

Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 kDa. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low and therefore beta Tubulin should not be used as loading control for these tissues.

Tubulin purified from polycephalum myxamoebae

Immunocytochemistry:
A 1-2 μg/mL concentration of a previous lot was used in immunocytochemistry.

Western blot:
0.05-0.5 μg/mL

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Tubulin Antibody, beta, clone KMX-1 is validated for use in IC, WB for the detection of Tubulin.

This antibody reacts specifically with β-tubulin and shows a strong cross-reactivity to different species. This antibody allows staining of microtubuli using indirect immunofluorescence showing a low background only.

Format: Purified

Protein A purified

Purified mouse monoclonal IgG2b liquid in buffer containing 0.02M phosphate buffer, 0.25M NaCl with 0.1% sodium azide.

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.

Control
HeLa Cell lysate

A431 Cell lysate

MCF7 Cell lysate

Routinely evaluated by Western Blot on A431 lysates.

Western blot:
1:500 dilution of this lot detected TUBULIN (BETA) on 10 μg of A431 lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1049

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.