콘텐츠로 건너뛰기
Merck

YEAST1

Yeast Transformation Kit

reagents for introducing plasmid DNA into yeast

동의어(들):

lithium acetate yeast transformation

조직 및 계약 가격을 보려면 로그인를 클릭합니다.

크기 선택

보기 변경

제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.85
UNSPSC Code:
12352200
Grade:
Molecular Biology
Technique(s):
transformation: suitable
기술 서비스
도움이 필요하신가요? 저희 숙련된 과학자 팀이 도와드리겠습니다.
도움 문의


grade

Molecular Biology

Quality Level

usage

 kit sufficient for >100 standard transformations

technique(s)

transformation: suitable

shipped in

dry ice

storage temp.

−20°C

General description

Sigma′s Yeast Transformation Kit contains all necessary reagents and controls for efficient transformation of yeast by the lithium acetate method.

Application

Suitable for transformation of any strain of yeast. Convenient, flexible and sensitive, positive transformants can be obtained with as little as 10 ng of DNA; the optimum efficiency is in the 0.1- 3 μg range.

Biochem/physiol Actions

Transformation with a plasmid complementing the mutated gene enables the transformant to grow on medium lacking the required component. Yeast cells are made competent for transformation by incubation in a buffered lithium acetate solution. Transformation is then carried out by incubating the cells together with transforming DNA and carrier DNA in a solution containing polyethylene glycol (PEG).

Features and Benefits

  • Easy and ready-to-use
  • Requires as little as 10 ng of plasmid DNA
  • Flexibility for any strain of yeast
  • Sufficient for over 100 standard transformations

Other Notes

The Yeast Transformation Kit contains:
  • Transformation Buffer; 100 ml; 100 mM lithium acetate, 10 mM Tris HCl, pH 7.6, and 1 mM EDTA
  • Plate Buffer; 100 ml; 40% PEG, 100 mM lithium acetate, 10 mM Tris HCl, pH 7.5, 1 mM EDTA
  • Deoxyribonucleic acid from salmon teste, 10 mg/ml; 2 x 1 ml
  • Control Yeast Plasmid DNA pRS316 carrying the ura gene; 10 μg
  • Yeast Synthetic Drop-out Medium Supplement Without Uracil; 1 g


키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS & 가격

  • Deoxyribonucleic acid, single stranded from salmon testes, For hybridization 2 x 1
    SDS

  • Yeast Synthetic Drop-out Medium Supplements, without uracil 1 g
    SDS

저장 등급

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3



가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문


프로토콜

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

문서

Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.

유전 공학 및 복제의 발전은 연구 목적을 위한 이형 Protein Expression Systems 및 분리에 수많은 가능성을 열어주었습니다. 상당한 수준의 기술 발전으로 재조합형 단백질의 대규모 발현과 분리가 가능하게 되었습니다.

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.


Improved method for high efficiency transformation of intact yeast cells.
D Gietz et al.
Nucleic acids research, 20(6), 1425-1425 (1992-03-25)
Tim R Blower et al.
Nucleic acids research, 47(15), 8163-8179 (2019-07-10)
Type II topoisomerases catalyze essential DNA transactions and are proven drug targets. Drug discrimination by prokaryotic and eukaryotic topoisomerases is vital to therapeutic utility, but is poorly understood. We developed a next-generation sequencing (NGS) approach to identify drug-resistance mutations in
Mechanistic and structural insights into the regioselectivity of an acyl-CoA fatty acid desaturase via directed molecular evolution.
Vanhercke T
The Journal of Biological Chemistry, 286(15), 12860-12869 (2011)



국제 무역 품목 번호

SKUGTIN
YEAST1-1KT04061837588167