grade
Molecular Biology
Quality Level
description
nutrient rich, quick dissolve, dust free formulation
sterility
non-sterile
form
powder
composition
Inert binder (EZMix only), 0.6 g/L , NaCl, 5 g/L , Tryptone (pancreatic digest of casein), 16 g/L , Yeast extract, 10 g/L
application(s)
agriculture
food and beverages
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Application
Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
2xYT EZMix powder provides:
- Granulated, dust-free format for safer handling and faster mixing
- Easy scale-up using larger package size
- Standard formulation
Preparation Note
Stir to suspend 31.6 g powder in 1L water. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Legal Information
저장 등급
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
프로토콜
General protocols for growth of competent cells in microbial medium.
관련 콘텐츠
Shuang Yang et al.
eLife, 9 (2020-06-09)
Dynamic trafficking of G protein-coupled receptors (GPCRs) out of cilia is mediated by the BBSome. In concert with its membrane recruitment factor, the small GTPase ARL6/BBS3, the BBSome ferries GPCRs across the transition zone, a diffusion barrier at the base