SRE0047
Proteinase K from Tritirachium album
Suitable for manufacturing of diagnostic kits and reagents, lyophilized powder, ≥30 units/mg protein, Molecular Biology
동의어(들):
Endopeptidase K
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크기 선택
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제품정보 (DICE 배송 시 비용 별도)
CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
254-457-8
MDL number:
eCl@ss:
32160410
Specific activity:
≥30 units/mg protein
Biological source:
fungus (Tritirachium album)
biological source
fungus (Tritirachium album)
Quality Level
grade
Molecular Biology
form
lyophilized powder
specific activity
≥30 units/mg protein
mol wt
28,930 Da, 28.93 kDa
storage condition
dry at room temperature
technique(s)
DNA extraction: suitable, RNA extraction: suitable
impurities
≤1 ppm DNA
color
white
pH
7.5
application(s)
diagnostic assay manufacturing
foreign activity
DNAse, Nickase and RNAse, none detected
shipped in
wet ice
storage temp.
−20°C
General description
Proteinase K is an extracellular endopeptidase. It is synthesized by the mold Tritirachium album Limber. Proteinase K belongs to a new subfamily of the subtilisins. It is a 277 amino acid protein with a molecular weight of 28,930 Da. Active proteinase K is characterized with an unhydrolysed protein chain and autolysed polypeptide chains.
Application
Proteinase K catalyzes the hydrolysis of keratin.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.
Biochem/physiol Actions
Proteinase K catalyzes the hydrolysis of keratin.
Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.
Features and Benefits
Features:
- Qualified Raw Materials: Products that meet the stringent requirements of the Elevated Assurance Program, ensuring compliance with regulatory standards.
- Comprehensive Documentation: Includes essential documents such as certificates, impurity profiles, and declarations to support audits and risk assessments.
- M-Clarity™ Quality Attributes: Products are classified within the MQ300 quality segment or higher, demonstrating superior quality assurance. M-Clarity™ Program
- Confirmed Process Controls: Established processes that ensure consistency and reliability in manufacturing.
- Enhanced Change Notification: Timely updates on any changes to product specifications or processes, ensuring transparency and compliance.
- Freely Available Dossiers: Access to robust, ready-to-present product dossiers that include:
- Site quality assessment
- ISO 9001 certificate
- Detailed product specifications
- Relevant declarations/statements (e.g., animal origin content, BSE/TSE, nitrosamine)
- Regulatory Compliance: Helps you meet compliance standards effectively, reducing the risk of regulatory issues.
- Audit Readiness: Instills confidence during audits with comprehensive documentation and confirmed process controls, demonstrating your commitment to high-quality products.
- Operational Transparency: Supports supply chain transparency, allowing you to track and verify the quality of raw materials.
- Enhanced Support: Provides exceptional quality-related information and tailored assistance to meet your specific needs.
- Risk Mitigation: Facilitates thorough risk assessments with detailed documentation, helping you manage potential compliance challenges.
- Informed Decision-Making: Access to clear and comprehensive product information enables better decision-making and enhances operational efficiency.
Other Notes
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Legal Information
M-CLARITY is a trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
저장 등급
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
문서
Balancing Proteinase K cost with quality and technical support ensures optimal enzyme selection for diverse applications.
Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.
In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.
Marin Volarić et al.
Genes, 12(8) (2021-08-28)
The long-read Nanopore sequencing has been recently applied for assembly of complex genomes and analysis of linear genome organization. The most critical factor for successful long-read sequencing is extraction of high molecular weight (HMW) DNA of sufficient purity and quantity.
Proteinase K from Tritirachium album Limber
Ebeling W, et al.
FEBS Journal, 47(1), 91-97 (1974)
Amino acid sequence of proteinase K from the mold Tritirachium album Limber: Proteinase K ? a subtilisin-related enzyme with disulfide bonds.
Jany KD, et al.
Febs Letters, 199(2), 139-144 (1986)
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| SRE0047-100MG | 04061832707815 |
| SRE0047-1G | 04061826650219 |
| SRE0047-500MG | 04061832707822 |