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Merck

S9751

Sulfatase from Helix pomatia

Type H-2, aqueous solution, ≥2,000 units/mL

동의어(들):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
UNSPSC Code:
12352204
NACRES:
NA.54
EC 번호:
MDL number:
Concentration:
≥2,000 units/mL
기술 서비스
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type

Type H-2

Quality Level

form

aqueous solution

secondary activity

≥100,000 units/mL β-glucuronidase (at pH 5.0)

mol wt

~85 kDa by gel filtration

concentration

≥2,000 units/mL

shipped in

wet ice

storage temp.

2-8°C

General description

Sequential action of sulfatase from Helix pomatia and β-O-glucosidase from Caldicellulosiruptor saccharolyticus on glucosinolates allows synthesis of thiohydroximates. Sulfatase from Helix pomatia hydrolyzes β-naphthyl sulfate much faster than α-naphthyl sulfate.

Application

Sulfatase from Helix pomatia is often used to remove sulfate groups from carbohydrates.

Other Notes

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

저장 등급

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Lot/Batch Number

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문서 라이브러리 방문


프로토콜

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)


Jakub Kopycki et al.
Biotechnology letters, 33(5), 1039-1046 (2011-01-27)
Thiohydroximates comprise a diverse class of compounds important in both biological and industrial chemistry. Their syntheses are generally limited to simple alkyl and aryl compounds with few stereocenters and a narrow range of functional groups. We hypothesized that sequential action
Abigail M Tokheim et al.
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 18(5), 537-540 (2005-12-08)
Often used to remove sulfate groups from carbohydrates, the regulatory properties of the aryl sulfatase from Helix pomatia remain little characterized. As many hydrolytic enzymes utilize exogenous metal ions in catalysis, the effect of various divalent metal ions on the
G Pelsy et al.
Biotechnology and bioengineering, 25(4), 919-928 (1983-04-01)
It has been found that sulfatase from Helix pomatia hydrolyzes beta-naphthyl sulfate much faster than alpha-naphthyl sulfate; e.g., at pH 7.8, while the former is readily hydrolyzed, the latter undergoes no appreciable hydrolysis. Kinetic investigations of both enzymatic and acid



국제 무역 품목 번호

SKUGTIN
S9751-25ML04061838256386
S9751-5ML04061836961961
S9751-10ML04061836961909