MOD50
Imprint® DNA Modification Kit
For bisulfite DNA conversion & purification
동의어(들):
Methylation-Specifc PCR preparation, bisulfite conversion kit, bisulphite modification
관련 카테고리
일반 설명
The Imprint® DNA Modification Kit contains all of the reagents necessary for complete bisulfite conversion and subsequent purification of DNA samples. DNA is chemically denatured to allow bisulfite reagent to react specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. Converted DNA is suitable for a variety of downstream applications including Methylation-Specifc PCR, methylation sequencing, and pyrosequencing, as well as methylation microarray.
애플리케이션
Imprint® DNA Modification Kit has been used for bisulfite modification of DNA.
특징 및 장점
- Only 50 picograms of DNA or 20 cells are required
- Procedure takes less than 2 hours
- Greater than 99% conversion rate
- Extremely low degradation
- Option of convenient one-step protocol
- Consistent and reproducible Bisulfite Modification
- Can be used with genomic, endonuclease digested, and FFPE DNA
제조 메모
All components can be stored at room temperature. Each vial of DNA Modification Powder is sufficient for ten DNA modifications. Once dissolved, the solution can be stored at -20 °C for one week, kept away from light. Before use, the frozen solution must be thawed at room temperature and vortexed for two minutes.
법적 정보
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
보충제 위험성
Storage Class Code
8B - Non-combustible corrosive hazardous materials
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
M Y Follo et al.
Leukemia, 26(5), 943-950 (2011-10-29)
Phosphoinositide-phospholipase C (PI-PLC) beta1 can be considered a specific target for demethylating therapy in high-risk myelodysplastic syndrome (MDS) patients, as azacitidine treatment has been associated with a PI-PLCbeta1-specific promoter demethylation, and induction of PI-PLCbeta1 gene and protein expression. However, little
Susanne Edelmann et al.
Methods in molecular biology (Clifton, N.J.), 1675, 45-60 (2017-10-21)
Bisulfite sequencing (BS-seq) enables the detection of DNA methylation at cytosine residues (5mC) at single-nucleotide resolution. For many applications, a limiting factor of conventional BS-seq protocols is the high amount of DNA required, since the treatment with bisulfite causes severe
Javed Hussain Choudhury et al.
Methods in molecular biology (Clifton, N.J.), 1726, 111-122 (2018-02-23)
DNA methylation plays a decisive role in the regulation and control of gene expression. DNA methylation is a covalent modification, in which a methyl group is attached to the 5th carbon of the cytosine ring of a CpG dinucleotide that
MicroRNA-20 induces methylation of hepatitis B virus covalently closed circular DNA in human hepatoma cells
In Young Moon, et al.
Molecular Medicine Reports, 2285-2293 (2019)
Histone H3 Lysine 36 Trimethylation Is Established over the Xist Promoter by Antisense Tsix Transcription and Contributes to Repressing Xist Expression
Ohhata T, et al.
Molecular and Cellular Biology null
문서
Bisulfite treatment distinguishes methylated DNA in gene methylation studies, exploiting the stability of methylated cytosine.
프로토콜
Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.
관련 콘텐츠
The Imprint DNA Modification Kit provides the reagents needed for bisulfite conversion and post-modification clean-up of DNA samples in less than 2 hours.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.